World Journal of Microbiology and Biotechnology

, Volume 26, Issue 2, pp 353–358

Agrobacteriumtumefaciens-mediated transformation of SOD gene to Trichoderma harzianum

  • Liming Yang
  • Qian Yang
  • Kening Sun
  • Ye Tian
  • Hulun Li
Original Paper

DOI: 10.1007/s11274-009-0182-4

Cite this article as:
Yang, L., Yang, Q., Sun, K. et al. World J Microbiol Biotechnol (2010) 26: 353. doi:10.1007/s11274-009-0182-4

Abstract

Trichoderma harzianum is a soil-borne filamentous fungus that exhibits biological control properties because it parasitizes a large variety of phytopathogenic fungi. In this study the SOD gene was successfully transferred into the bio-control fungus Trichoderma harzianum with an efficiency of 60–110 transformants per 107 spores by using Agrobacterium tumefaciens-mediated transformation. Putative transformants were analyzed to test the transformation by the southern blot. Antifungal activities of the transformants were examined under abiotic stresses. The transformants were exposed to 40°C for three days and 2 mol/l NaCl at 27°C for 5–10 days to assay antifungal activities with Sclerotinia sclerotiorum. The inhibition rates of the transformants, comparing to Trichoderma harzianum with no SOD gene transferred, were respectively 83.96% after 40°C and 60.13% after 2 mol/l NaCl. The results showed that the SOD transformants had significantly higher resistance to heat and salt stress.

Keywords

Trichoderma harzianum Biocontrol Agrobacterium tumefaciens Abiotic stresses 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Liming Yang
    • 1
    • 2
    • 4
  • Qian Yang
    • 4
  • Kening Sun
    • 2
  • Ye Tian
    • 1
  • Hulun Li
    • 3
  1. 1.Department of PathophysiologyHarbin Medical UniversityHarbinChina
  2. 2.Department of Applied ChemistryHarbin Institute of TechnologyHarbinChina
  3. 3.Department of NeurobiologyHarbin Medical UniversityHarbinChina
  4. 4.Department of Life Science and EngineeringHarbin Institute of TechnologyHarbinChina

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