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World Journal of Microbiology and Biotechnology

, Volume 26, Issue 2, pp 359–363 | Cite as

Isolation and characterization of Pseudomonas sp. KUMS3 from Asian sea bass (Lates calcarifer) with fin rot

  • S. KumaranEmail author
  • B. DeivasigamaniEmail author
  • K. M. Alagappan
  • M. Sakthivel
  • S. Guru Prasad
Short Communication

Abstract

Bacteria were isolated from Asian Sea bass, Lates calcarifer kept in a farm, on the South-east coast of India. During an outbreak of fin rot, the affected fish had hemorrhages at the base of fins, mouth and skin muscles and faded pigments. Pure colonies were isolated on NA and ZMA from internal organs of the fish and the bacterial morphology was identified as gram-negative rod-shaped bacteria. Based on different biochemical tests and sequence of 16S rDNA, the causative bacteria were identified as Pseudomonas sp. KUMS3. Bacterial cells were isolated from liver and kidney of all artificially infected moribund fish and confirmed as Pseudomonas sp. KUMS3 by morphological and biochemical characteristics. During the experimental infection, the first incidence of dead fish was observed on 2nd day after exposure to Pseudomonas sp. KUMS3 and no fish died after 12 days post exposure and the cumulative percent of mortality was 70. Histological lesions were observed in the spleen, liver and kidney of the infected fish. Pseudomonas sp. KUMS3 could be considered as an opportunistic pathogen, which can survive on the fish surface or in water or in the gut and may cause disease when unfavorable conditions develop.

Keywords

Sea bass Disease Hemorrhages Fin rot Infection Pseudomonas sp. 

References

  1. Agbede SA, Adeyemo OK, Adedeji O, Junaid AU (2005) Ultrastructural study of the phagocytic activities of splenic macrophages in tilapia (Oreochromis niloticus). Afr J Biotechnol 5(22):2350–2353Google Scholar
  2. Ahne W, Popp W, Hoffmann R (1982) Pseudomonas fluorescens as a pathogen of tench (Tinca tinca). Bull Eur Assoc Fish Pathol 4:56–57Google Scholar
  3. Altinok I, Kayis S, Capkin E (2006) Pseudomonas putida infection in rainbow trout. Aquaculture 261:850–855CrossRefGoogle Scholar
  4. Altinok I, Balta F, Capkin E, Kayis S (2007) Disease of rainbow trout caused by Pseudomonas luteola. Aquaculture 273:393–397CrossRefGoogle Scholar
  5. Angelini NM, Seigneur GN (1988) Disease of the fins of Rhamdia sapo. Isolation of the etiological agents and experimental infection. Rev Argent Microbiol 20:37–48Google Scholar
  6. Austin B, Austin DA (1999) Bacterial fish pathogens: disease of farmed and wild fish, 3rd edn. Praxis Publishing Ltd, Chichester, pp 29–32 (reviesed)Google Scholar
  7. Bachere E, Mialhe E, Noel D et al (1995) Knowledge and research prospects in marine mollusc and crustacean immunology. Aquaculture 132:17–32CrossRefGoogle Scholar
  8. Berthe FCJ, Michel C, Bernardet JF (1995) Identification of Pseudomonas anguilliseptica isolated from several fish species in France. Dis Aquat Org 21:151–155CrossRefGoogle Scholar
  9. Bullock GL (1965) Characteristics and pathogenicity of a capsulated Pseudomonas isolated from goldfish. Appl Microbiol 13:89–92Google Scholar
  10. Deviasigamani B (2007) Structure of immune organ in edible catfish, Mystus gulio. J Environ Biol 28:757–764Google Scholar
  11. Greenwood PH (1976) A review of the family centropomidae (Pisces perciformes). Bull Br Mus Nat Hist (Zoology) 29:1–81Google Scholar
  12. Guomin Q, Xiaojun Z, Cuizhen C et al (2007) Identification and characterization of pathogen to bacterial septicaemia in cultured turbot, Scophthalmus maximus. Chin J Oceanol Limnol 25(4):403–410CrossRefGoogle Scholar
  13. Hatai K, Egusa S, Nakajima M, Chikahata H (1975) Pseudomonas chlororaphis as a fish pathogen. Bull Jpn Soc Sci Fish 41:1203Google Scholar
  14. Li MF, Jordan C (1968) A proteolytic pseudomonad from skin lesions of rainbow trout (Salmo Gairdneri). II. Some properties of the proteinase. Can J Microbiol 14:875–880CrossRefGoogle Scholar
  15. Liu PC, Lin JY, Chuang WH, Lee KK (2004) Isolation and characterization of pathogenic Vibrio harveyi (V. carchariae) from the farmed marine cobia fish Rachycentron canadum L. with gastroenteritis syndrome. World J Microbiol Biotechnol 20:495–499CrossRefGoogle Scholar
  16. Lonnstrom L, Wiklund T, Bylund G (1994) Pseudomonas anguilliseptica isolated from Baltic herring Clupea harengus membras with eye lesions. Dis Aquat Org 18:143–147CrossRefGoogle Scholar
  17. Muroga K, Jo Y, Sawada T (1975) Studies on red spot disease of pond cultured eels. Part II. Pathogenicity of the causative bacterium Pseudomonas anguilliseptica. Fish pathol 9:107–114Google Scholar
  18. Saeed MO, Alomoundi MM, Al-Harbi AH (1987) A pseudomonas associated with disease in cultured rabbit fish (Siganus rivulatus) in the Red sea. Dis Aquat Org 3:177–180CrossRefGoogle Scholar
  19. Soffientino B, Gwaltney T, Nelson DR, Specker JL, Mauel M, Gomez-Chiarri M (1999) Infectious necrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture. Dis Aquat Org 38:201–210CrossRefGoogle Scholar
  20. Tucker JW, Russell DJ, Rimmer MA (2002) Barramundi culture: a success story for aquaculture in Asia and Australia. World Aquac 33:53–59Google Scholar
  21. Verschuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671CrossRefGoogle Scholar
  22. Wakabayashi H, Egusa S (1972) Characteristics of Pseudomonas sp. from an epizootic of pond- cultured eels (Anguilla japonica). Bull Jpn Soc Sci Fish 38:577–587Google Scholar
  23. Wiklund T, Bylund G (1993) Skin ulcer disease of flounder Platichithys flesus in the northern Baltic sea. Dis Aquat Org 17:165–174CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Centre of Advanced Study in Marine BiologyAnnamalai UniversityParangipettaiIndia

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