Isolation and characterization of Pseudomonas sp. KUMS3 from Asian sea bass (Lates calcarifer) with fin rot
- 352 Downloads
Bacteria were isolated from Asian Sea bass, Lates calcarifer kept in a farm, on the South-east coast of India. During an outbreak of fin rot, the affected fish had hemorrhages at the base of fins, mouth and skin muscles and faded pigments. Pure colonies were isolated on NA and ZMA from internal organs of the fish and the bacterial morphology was identified as gram-negative rod-shaped bacteria. Based on different biochemical tests and sequence of 16S rDNA, the causative bacteria were identified as Pseudomonas sp. KUMS3. Bacterial cells were isolated from liver and kidney of all artificially infected moribund fish and confirmed as Pseudomonas sp. KUMS3 by morphological and biochemical characteristics. During the experimental infection, the first incidence of dead fish was observed on 2nd day after exposure to Pseudomonas sp. KUMS3 and no fish died after 12 days post exposure and the cumulative percent of mortality was 70. Histological lesions were observed in the spleen, liver and kidney of the infected fish. Pseudomonas sp. KUMS3 could be considered as an opportunistic pathogen, which can survive on the fish surface or in water or in the gut and may cause disease when unfavorable conditions develop.
KeywordsSea bass Disease Hemorrhages Fin rot Infection Pseudomonas sp.
- Agbede SA, Adeyemo OK, Adedeji O, Junaid AU (2005) Ultrastructural study of the phagocytic activities of splenic macrophages in tilapia (Oreochromis niloticus). Afr J Biotechnol 5(22):2350–2353Google Scholar
- Ahne W, Popp W, Hoffmann R (1982) Pseudomonas fluorescens as a pathogen of tench (Tinca tinca). Bull Eur Assoc Fish Pathol 4:56–57Google Scholar
- Angelini NM, Seigneur GN (1988) Disease of the fins of Rhamdia sapo. Isolation of the etiological agents and experimental infection. Rev Argent Microbiol 20:37–48Google Scholar
- Austin B, Austin DA (1999) Bacterial fish pathogens: disease of farmed and wild fish, 3rd edn. Praxis Publishing Ltd, Chichester, pp 29–32 (reviesed)Google Scholar
- Bullock GL (1965) Characteristics and pathogenicity of a capsulated Pseudomonas isolated from goldfish. Appl Microbiol 13:89–92Google Scholar
- Deviasigamani B (2007) Structure of immune organ in edible catfish, Mystus gulio. J Environ Biol 28:757–764Google Scholar
- Greenwood PH (1976) A review of the family centropomidae (Pisces perciformes). Bull Br Mus Nat Hist (Zoology) 29:1–81Google Scholar
- Hatai K, Egusa S, Nakajima M, Chikahata H (1975) Pseudomonas chlororaphis as a fish pathogen. Bull Jpn Soc Sci Fish 41:1203Google Scholar
- Muroga K, Jo Y, Sawada T (1975) Studies on red spot disease of pond cultured eels. Part II. Pathogenicity of the causative bacterium Pseudomonas anguilliseptica. Fish pathol 9:107–114Google Scholar
- Tucker JW, Russell DJ, Rimmer MA (2002) Barramundi culture: a success story for aquaculture in Asia and Australia. World Aquac 33:53–59Google Scholar
- Wakabayashi H, Egusa S (1972) Characteristics of Pseudomonas sp. from an epizootic of pond- cultured eels (Anguilla japonica). Bull Jpn Soc Sci Fish 38:577–587Google Scholar