Solid-state fermentation of cornmeal with the ascomycete Morchella esculenta for degrading starch and upgrading nutritional value

  • Gui-Ping Zhang
  • Feng Zhang
  • Wen-Ming Ru
  • Jian-Rong HanEmail author
Original Paper


The ability of the ascomycete Morchella esculenta to degrade starch and upgrade nutritional value of cornmeal during solid-state fermentation (SSF) was studied. On the basal medium, α-amylase activity of M. esculenta reached its maximum value of 215 U g−1 of culture on day 20 after inoculation. Supplementation of glucose, yeast extract to the basal medium caused a significant increase in either the degradation rate of starch or the mycelial biomass as compared with control (P < 0.01). Through orthogonal experiments, the theoretical optimum culture medium for SSF of this fungus was the following: 100 g cornmeal, ground to 30-mesh powder, moistened with 67 ml of nutrient salt solution supplemented with 3 g yeast extract and 10 g glucose per liter. Under the optimum culture condition, the degradation rate of starch reached its maximum values of 74.8%; the starch content of the fermented product decreased from 64.5 to 23.5%.


Cornmeal Morchella esculenta Mycelial biomass Solid-state fermentation Starch 



Support for this research by the Shanxi Province Science Foundation (No. 2008012010-2) is gratefully acknowledged.


  1. Amir R, Levanon D, Hadar Y et al (1993) Morphology and physiology of Morchella esculenta during sclerotial formation. Mycol Res 97:683–689CrossRefGoogle Scholar
  2. Bernfeld P (1955) Alpha-amylase. In: Colowick S, Kaplan NO (eds) Methods in enzymology. Academic Press, New York, pp 149–152CrossRefGoogle Scholar
  3. Charcosset JY, Chauvet E (2001) Effect of culture conditions on ergosterol as an indicator of biomass in the aquatic hyphomycetes. Appl Environ Microbiol 67:2051–2055CrossRefGoogle Scholar
  4. Du RJ (1985) Biological statistics. Higher Education Press, BeijingGoogle Scholar
  5. Gilbert FA (1960) The submerged culture of Morchella. Mycologia 52:201–209CrossRefGoogle Scholar
  6. Güler P, Arkan O, Erik S (1996) Cultural characteristics of Morchella conica Pers. On the some nutritious. Tr J Biol 20:75–86Google Scholar
  7. Han JR (2003) Solid-state fermentation of corn meal with the basidiomycete Hericium erinaceum for degrading starch and upgrading nutritional value. Int J Food Microbiol 80:61–66CrossRefGoogle Scholar
  8. Hervey AG, Bistis G, Leong I (1978) Cultural studies of single ascospore isolates of Morchella esculenta. Mycologia 70:1269–1274CrossRefGoogle Scholar
  9. Hilliam M (1998) The market for functional foods. Int Dairy J 8:349–353CrossRefGoogle Scholar
  10. Huang WK (1989) Examination and analysis of food. China Light Industry Press, BeijingGoogle Scholar
  11. Janardhanan KK, Kaul TN, Husain A (1977) Use of vegetable wastes for the production of fungal protein from Morchella species. J Food Sci Technol 7:197–199Google Scholar
  12. Lin CN, Tome WP, Won SJ (1991) Novel cytotoxic principles of Formosan Ganoderma lucidum. J Nat Prod 54:998–1002CrossRefGoogle Scholar
  13. Litchfield JH (1955) Submerged culture of mushroom mycelium. In: Peppler HJ (ed) Microbial technology. Reinhold Publishing Corp, New York, pp 107–144Google Scholar
  14. Matcham SE, Jordan BR, Wood DA (1985) Estimation of fungal biomass in a solid substrate by three independent methods. Appl Microbiol Biotechnol 21:108–112CrossRefGoogle Scholar
  15. Mau JL, Chang CN, Huang SJ et al (2004) Antioxidant properties of methanolic extracts from Grifola frondosa, Morchella esculenta and Termitomyces albuminosus mycelia. Food Chem 87:111–118CrossRefGoogle Scholar
  16. Pandey A, Soccol CR, Mitchell D (2000) New developments in solid-state fermentation I. Bioprocesses and products. Process Biochem 35:1153–1169CrossRefGoogle Scholar
  17. Phillips R (1991) Mushrooms of North America. Little Brown and Company, Boston, MAGoogle Scholar
  18. Royse DJ, May B (1990) Interspecific allozyme variation among Morchella spp and its inferences for systematics within the genus. Biochem Syst Ecology 18:475–479CrossRefGoogle Scholar
  19. Weng CC (2003) Taste quality of Grifola frondosa, Morchella esculenta and Termitomyces albuminosus mycelia and their application in food processing. In: Master’s Thesis. National Chung-Hsing University, TaiwanGoogle Scholar
  20. Yang JX, Tao SX, Song TS (1992) Improvement of nutritional value in enzymatically hydrolyzed cornmeal by Candida tropicalis. Acta Nutrimenta Sinica 14:182–185Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Gui-Ping Zhang
    • 1
    • 2
  • Feng Zhang
    • 1
  • Wen-Ming Ru
    • 2
  • Jian-Rong Han
    • 3
    Email author
  1. 1.Institute of Loess PlateauShanxi UniversityTaiyuanChina
  2. 2.Department of Biology & ChemistryChangzhi CollegeChangzhiChina
  3. 3.School of Life Science and TechnologyShanxi UniversityTaiyuanChina

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