Morphological changes of Klebsiella pneumoniae in response to Cefotaxime: a scanning electron microscope study

  • H. Rajeshwari
  • S. Nagveni
  • Ajay Oli
  • Deepti Parashar
  • Kelmani R. ChandrakanthEmail author
Short Communication


Multi drug resistant Klebsiella pneumoniae showed stepwise adaptation when grown in increasing concentration of Cefotaxime eventually reaching a maximum of 2 mg/ml. The resultant Cefotaxime resistant mutant strain was stable and did not revert to susceptibility on frequent subculturing. The response of the cells to different concentration of Cefotaxime was examined by scanning electron microscope which showed that the size of the bacterium increased with increasing concentration of Cefotaxime.


K. pneumoniae Morphological changes Step-wise adaptation Cefotaxime Scanning electron microscopy 



We would like to Thank Miss. Sushmita Potluri for her help in Scanning electron microscopy and Dr. P. M. Nimbargi, Department of microbiology, Gulbarga University, Gulbarga for his vocabulary and manuscript review and comments.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • H. Rajeshwari
    • 1
  • S. Nagveni
    • 1
  • Ajay Oli
    • 1
  • Deepti Parashar
    • 2
  • Kelmani R. Chandrakanth
    • 1
    Email author
  1. 1.Department of BiotechnologyGulbarga UniversityGulbargaIndia
  2. 2.National Institute of VirologyPuneIndia

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