Advertisement

Morphological changes of Klebsiella pneumoniae in response to Cefotaxime: a scanning electron microscope study

  • H. Rajeshwari
  • S. Nagveni
  • Ajay Oli
  • Deepti Parashar
  • Kelmani R. ChandrakanthEmail author
Short Communication

Abstract

Multi drug resistant Klebsiella pneumoniae showed stepwise adaptation when grown in increasing concentration of Cefotaxime eventually reaching a maximum of 2 mg/ml. The resultant Cefotaxime resistant mutant strain was stable and did not revert to susceptibility on frequent subculturing. The response of the cells to different concentration of Cefotaxime was examined by scanning electron microscope which showed that the size of the bacterium increased with increasing concentration of Cefotaxime.

Keywords

K. pneumoniae Morphological changes Step-wise adaptation Cefotaxime Scanning electron microscopy 

Notes

Acknowledgments

We would like to Thank Miss. Sushmita Potluri for her help in Scanning electron microscopy and Dr. P. M. Nimbargi, Department of microbiology, Gulbarga University, Gulbarga for his vocabulary and manuscript review and comments.

References

  1. Aldsworth TG, Sharman RL, Dodd CER (1999) Bacterial suicide through stress. Cell Mol Life Sci 56:378–383. doi: 10.1007/s000180050439 CrossRefGoogle Scholar
  2. Dodd CER, Sharman RL, Bloomfied SF, Booth IR, Stewaet GSAB (1999) Inimical processes: bacterial self-distraction and sublethal injury. Trends Food Sci Technol 8:238–241. doi: 10.1016/S0924-2244(97)01043-1 CrossRefGoogle Scholar
  3. Heipieper HJ, Weber FJ, Sikkema J, Keweloh H, de Bont JAM (1993) Mechanism behind resistance of whole cells to toxic organic solvents. Trends Biotechnol 12:409–415. doi: 10.1016/0167-7799(94)90029-9 CrossRefGoogle Scholar
  4. Kessel RG, Shib CY (1974) Scanning electron microscopy in biology. Spring-Verlag, Berlin, Heidelberg, New YorkGoogle Scholar
  5. Neumann G, Veerangouda Y, Karegoudar TB, Sahin O, Mausezahl I, Kabelitz N, Kappelmeyer U, Heipieper HJ (2005) Cells of pseudomonas putida and Enterobacter sp. adapt to toxic organic compounds by increasing their size. Extermophiles 9:163–168. doi: 10.1007/s00792-005-0431-x CrossRefGoogle Scholar
  6. O’Hara GW, Glenn AR (1994) The adaptive acid tolerance response in root nodule bacteria and Escherichia coli. Arch Microbiol 161:286–292. doi: 10.1007/BF00303582 CrossRefGoogle Scholar
  7. Ritz M, Tholozan JL, Federighi M, Pilet MF (2001) Morphological and physiological characterization of Listeria monocytogenes subjected to high hydrostatic pressure. Appl Environ Microbiol 67:2240–2247. doi: 10.1128/AEM.67.5.2240-2247.2001 CrossRefGoogle Scholar
  8. Shi B, Xia X (2003) Morphological changes of pseudomonas pseudoalcaligenes in response to temperature selection. Curr Microbiol 46:120–123. doi: 10.1007/s00284-002-3824-4 CrossRefGoogle Scholar
  9. Thomas LV, Wimpenny JWT (1996) Investigation of the effect of combined variations in temperature, pH and NaCl concentrations on nisin inhibition of Listeria monocytogenes and Staphylococcus aureus. Appl Environ Microbiol 62:2006–2012Google Scholar
  10. Zanetti M, Gennaro R, Skerlavaj B et al (2002) Cathelicidin peptides as candidates for a novel class of antimicrobials. Curr Pharm Des 8:779–793. doi: 10.2174/1381612023395457 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • H. Rajeshwari
    • 1
  • S. Nagveni
    • 1
  • Ajay Oli
    • 1
  • Deepti Parashar
    • 2
  • Kelmani R. Chandrakanth
    • 1
    Email author
  1. 1.Department of BiotechnologyGulbarga UniversityGulbargaIndia
  2. 2.National Institute of VirologyPuneIndia

Personalised recommendations