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Isolation and partial characterization of alkaline feruloyl esterases from Aspergillus niger CFR 1105 grown on wheat bran

Abstract

Feruloyl esterases (FAEs) of a strain of Aspergillus niger (CFR 1105) grown in solid state (ssf) and submerged fermentations (smf) using wheat bran both as carbon source and inducer of the enzyme were studied. The feruloyl esterase activity was maximum after 4 days in solid state as well as in submerged fermentations (32.5 and 31.5 U/g dry weight of wheat bran respectively) and the enzyme titers were comparable. The specific activity was maximum on day 2 in ssf (12.8 U/mg protein) and it decreased thereafter, whereas specific activity was maximum on day 3 (11.7 U/mg protein) in smf and it remained constant up to 5 days. Two isoenzymes of feruloyl esterases were isolated and purified to homogeneity by conventional protein purification methods from the day 5 culture filtrate of A. niger grown in smf. On a DEAE-cellulose column, two enzyme activity peaks designated as FAE-1 and FAE-2 were eluted with 0.3 and 0.35 M NaCl, respectively. They were monomeric glycoproteins with approximate molecular weights of 50 kDa (FAE-1) and 55 kDa (FAE-2), respectively. FAE-1 showed a temperature optimum of 40°C whereas FAE-2 showed a wider temperature optimum of 40–50°C. FAE-1 and FAE-2 exhibited pH optima of 9 and 6, respectively, and both were stable over a pH range of 6–9. The ability of the enzyme to be active in alkaline pH may be advantageous in biotechnological applications.

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Acknowledgments

We thank Dr. V. Prakash, F.R.Sc., Director CFTRI for his constant support and encouragement and also thank Dr. M. C. Varadaraj Head, Human Resource Development, CFTRI for providing laboratory facilities for microbiological experiments. SH thanks Council of Scientific & Industrial Research, New Delhi, India for the grant of Senior Research Fellowship.

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Correspondence to Gudipati Muralikrishna.

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Hegde, S., Muralikrishna, G. Isolation and partial characterization of alkaline feruloyl esterases from Aspergillus niger CFR 1105 grown on wheat bran. World J Microbiol Biotechnol 25, 1963–1969 (2009). https://doi.org/10.1007/s11274-009-0095-2

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Keywords

  • Solid state fermentation
  • Submerged fermentation
  • Aspergillus niger CFR 1105
  • Feruloyl esterase
  • Wheat bran