World Journal of Microbiology and Biotechnology

, Volume 25, Issue 11, pp 1929–1939 | Cite as

Optimization of amylase production by Aspergillus niger in solid-state fermentation using sugarcane bagasse as solid support material

Original Paper

Abstract

Synthesis of amylase by Aspergillus niger strain UO-01 under solid-state fermentation with sugarcane bagasse was optimized by using response surface methodology and empirical modelling. The process parameters tested were particle size of sugarcane bagasse, incubation temperature and pH, moisture level of solid support material and the concentrations of inoculum, total sugars, nitrogen and phosphorous. The optimum conditions for high amylase production (457.82 EU/g of dry support) were particle size of bagasse in the range of 6–8 mm, incubation temperature and pH: 30.2°C and 6.0, moisture content of bagasse: 75.3%, inoculum concentration: 1 × 107 spores/g of dry support and concentrations of starch, yeast extract and KH2PO4: 70.5, 11.59 and 9.83 mg/g of dry support, respectively. After optimization, enzyme production was assayed at the optimized conditions. The results obtained corroborate the effectiveness and reliability of the empirical models obtained.

Keywords

Amylase Optimization Solid-state fermentation Sugarcane bagasse 

Abbreviations

RSM

Response surface methodology

SLF

Submerged liquid fermentation

SSF

Solid-state fermentation

gds

g of dry support

TAA

Total amylase activity

EU

Enzymatic units

T

Temperature

IC

Inoculum concentration

M

Moisture content

TS

Total sugars concentration

N

Nitrogen concentration

P

Phosphorous concentration

References

  1. Abu EA, Ado SA (2004) Comparative studies on the effect of organic and inorganic nitrogen supplementation of millet and sorghum pomace on the production of three industrial enzymes by Aspergillus niger SL.1. Biokemistry 16:64–70Google Scholar
  2. Ali S, Sayed A, Sarker RT, Alam R (1991) Factors affecting cellulose production by Aspergillus terreus using water hyacinth. World J Microbiol Biotechnol 7:62–66Google Scholar
  3. Balkan B, Ertan F (2007) Production of α-amylase from P. chrysogenum. Food Technol Biotechnol 45:439–442Google Scholar
  4. Baysal Z, Uyar F, Aytekin C (2003) Solid state fermentation for production of α-amylase by a thermotolerant Bacillus subtilis from hot-spring water. Process Biochem 38:1665–1668CrossRefGoogle Scholar
  5. Bhargav S, Panda BP, Ali M, Javed S (2008) Solid-state fermentation: an overview. Chem Biochem Eng Q 22:49–70Google Scholar
  6. Doran JB, Aldrich HC, Ingram LO (1994) Saccharification and fermentation of sugarcane bagasse. Biotechnol Bioeng 44:240–247CrossRefGoogle Scholar
  7. Ellaiah P, Adinarayana K, Bhavani Y, Padmaja P, Srinivasulu B (2002) Optimization of process parameters for glucoamylase production under solid state fermentation by a new isolated Aspergillus species. Process Biochem 38:615–620CrossRefGoogle Scholar
  8. Francis F, Sabu A, Nampoothiri KM, Szakacs G, Pandey A (2002) Synthesis of α-amylase by Aspergillus oryzae in solid-state fermentation. J Basic Microbiol 5:320–326CrossRefGoogle Scholar
  9. Francis F, Sabu A, Nampoothiri KM, Ramachandran S, Ghosh S, Szakacs G, Pandey A (2003) Use of response surface methodology for optimizing process parameters for the production of α-amylase by Aspergillus oryzae. Biochem Eng J 15:107–115Google Scholar
  10. Gigras P, Sahai V, Grupta R (2002) Statistical media optimization and production of its ITS alpha amylase from Aspergillus oryzae in a biorreactor. Curr Microbiol 45:203–208CrossRefGoogle Scholar
  11. Guerra NP, Pastrana L (2002) Nisin and pediocin production on mussel-processing waste supplemented with glucose and five nitrogen sources. Lett Appl Microbiol 34:114–118CrossRefGoogle Scholar
  12. Guerra NP, Torrado A, López C, Pastrana L (2003) Main characteristics and applications of solid substrate fermentation. Electron J Environ Agric Food Chem 2: 343–350. Available from: http://ejeafche.uvigo.es
  13. Gutiérrez-Correa M, Tengerdy RP (1997) Production of cellulase on sugarcane bagasse by fungal mixed culture solid substrate fermentation. Biotechnol Lett 19:665–667CrossRefGoogle Scholar
  14. Kashyap P, Sabu A, Pandey A, Szakacs G (2002) Extra-cellular l-glutaminase production by Zygosaccharomyces rouxii under solid-state fermentation. Process Biochem 38:307–312CrossRefGoogle Scholar
  15. Kekos D, Galiotou-Panayotou M, Macris BJ (1987) Some nutritional factors affecting α-amylase production by Calvatia gigantea. Appl Microbiol Biotechnol 26:527–530CrossRefGoogle Scholar
  16. Kunamneni A, Permaul K, Singh S (2005) Amylase production in solid state fermentation by the thermophilic fungus Thermomyces lanuginosus. J Biosci Bioeng 100:168–171CrossRefGoogle Scholar
  17. Laxmi GS, Sathish T, Rao CS, Brahmaiah P, Hymavathi M, Prakasham RS (2008) Palm fiber as novel substrate for enhanced xylanase production by isolated Aspergillus sp. RSP-6. Curr Trends Biotechnol Pharm 2:447–455Google Scholar
  18. Lonsane BK, Ghildyal NP, Budiatman S, Ramakrishna SV (1985) Engineering aspects of solid state fermentation. Enzyme Microb Technol 7:258–265CrossRefGoogle Scholar
  19. Mazutti M, Bender JP, Treichel H, Di Luccio M (2006) Optimization of inulinase production by solid-state fermentation using sugarcane bagasse as substrate. Enzyme Microb Technol 39:56–59CrossRefGoogle Scholar
  20. Mazutti M, Ceni G, Di Luccio M, Treichel H (2007) Production of inulinase by solid-state fermentation: effect of process parameters on production and preliminary characterization of enzyme preparations. Bioprocess Biosyst Eng 30:297–304CrossRefGoogle Scholar
  21. Michelena VV, Castillo FJ (1984) Production of amylase by Aspergillus foetidus on rice flour medium and characterization of the enzyme. J Appl Bacteriol 56:395–407Google Scholar
  22. Murado MA, Siso MIG, González MP, Montemayor MI, Pastrana L, Mirón J (1993) Characterization of microbial biomasses and amylolytic preparations obtained from mussel-processing waste treatment. Bioresour Technol 43:117–125CrossRefGoogle Scholar
  23. Murado MA, González MP, Torrado A, Pastrana LM (1997) Amylase production by solid state culture of Aspergillus oryzae on polyurethane foams. Some mechanistic approaches from an empirical model. Process Biochem 32:35–42CrossRefGoogle Scholar
  24. Pandey A (2003) Solid state fermentation. Biochem Eng J 13:81–84CrossRefGoogle Scholar
  25. Pandey A, Selvakumar P, Soccol CR, Nigam P (1999) Solid state fermentation for the production of industrial enzymes. Curr Sci 77:149–162Google Scholar
  26. Pandey A, Soccol CR, Nigam P, Soccol VT (2000) Biotechnological potential of agro-industrial residues. I: sugarcane bagasse. Bioresour Technol 74:69–80CrossRefGoogle Scholar
  27. Raimbault M (1998) General and microbiological aspects of solid substrate fermentation. Electron J Biotechnol 1: 1–22. Available from http://www.ejb.org/content/vol1/issue3/full/9/index.html
  28. Ramachandran S, Patel AK, Nampoothiri KM, Francis F, Nagy V, Szakacs G, Pandey A (2004) Coconut oil cake-a potential raw material for the production of α-amylase. Bioresour Technol 93:169–174CrossRefGoogle Scholar
  29. Rodríguez S, Sanromán MA (2006) Application of solid-state fermentation to food industry-A review. J Food Eng 76:291–302CrossRefGoogle Scholar
  30. Salas M, Rodríguez M, Guerra NP, Pérez R (2006) Amylase production by Aspergillus niger in submerged cultivation on two wastes from food industries. J Food Eng 73:93–100CrossRefGoogle Scholar
  31. Selvakumar P, Pandey A (1999) Solid state fermentation for the synthesis of inulinase from Staphylococcus sp. and Kluyveromyces marxianus. Process Biochem 34:851–858CrossRefGoogle Scholar
  32. Spier MR, Woiciechowski AL, Vandenberghe LPS, Zoclo CR (2006) Production and characterization of amylases by Aspergillus niger under solid state fermentation using agro industrials products. Int J Food Eng 2. Available from: http://www.bepress.com/ijfe/vol2/iss3/art6/
  33. Uma Maheswar Rao JL, Satyanarayana T (2007) Improving production of hyperthermostable and high maltose-forming α-amylase by an extreme thermophile Geobacillus thermoleovorans using response surface methodology and its applications. Bioresour Technol 98:345–352CrossRefGoogle Scholar
  34. Zadrazil F, Brunnert H (1981) Investigation of physical parameters important for the SSF of straw by white rot fungi. Eur J Appl Microbiol Biotechnol 11:183–188CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Pharmacy, Natural Science FacultyUniversity of OrienteSantiago de CubaCuba
  2. 2.Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Food Science and Technology Faculty, Ourense CampusUniversity of VigoOurenseSpain

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