World Journal of Microbiology and Biotechnology

, Volume 25, Issue 10, pp 1821–1828 | Cite as

Synergistic biodegradation of pentachlorophenol by Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385)

  • Shail Singh
  • B. B. Singh
  • R. Chandra
  • D. K. Patel
  • V. Rai
Original Paper


The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385) were used for pentachlorophenol (PCP) degradation. The consortia showed better overall removal efficiencies than single strains by utilization of PCP as a carbon and energy source confirmed by pH dependent dye indicator bromocresol purple (BCP) in mineral salt media (MSM). Mixed culture was found to degrade up to 93% of PCP (300 mg/l) as compared to single strains (62.75–90.33%), at optimized conditions (30 ± 1°C, pH 7 ± 0.2, 120 rpm) at 168 h incubation. PCP degradation was also recorded at 20°C (62.75%) and 37°C (83.33%); pH 6 (70%) and pH 9 (75.16%); 50 rpm (73.33%) and 200 rpm (91.63%). The simultaneous release of chloride ion up to 90.8 mg/l emphasized the bacterial dechlorination in the medium. GC–MS analysis revealed the formation of low molecular weight compound, i.e., 6-chlorohydroxyquinol, 2,3,4,6-tetrachlorophenol and tetrachlorohydroquinone, from degraded sample as compared to control.


Pentachlorophenol Degradation Dechlorination GC–MS 



Authors are thankful to Director, ITRC, Lucknow, for his encouragement. The financial support from Council of Science and Technology, U.P. is also highly acknowledged.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Shail Singh
    • 1
  • B. B. Singh
    • 1
  • R. Chandra
    • 1
  • D. K. Patel
    • 2
  • V. Rai
    • 3
  1. 1.Environmental Microbiology SectionIndian Institute of Toxicology ResearchLucknowIndia
  2. 2.Analytical Chemistry SectionIndian Institute of Toxicology ResearchLucknowIndia
  3. 3.School of Life SciencesPandit Ravi Shankar Shukla UniversityRaipurIndia

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