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Molecular mapping of rice blast resistance gene Pi-1(t) in the elite indica variety Samba mahsuri

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Abstract

Samba mahsuri (BPT 5204) is a cultivar of the medium slender grain indica variety of Oryza sativa grown across India for its high yield and quality. However, this cultivar is susceptible to several diseases and pests including rice blast. The analysis of near isogenic lines indicated the presence of a resistance gene, Pi-1(t) in the donor cultivar C101LAC which is highly resistant to the rice blast fungus Magnaporthe grisea (M. grisea). C101LAC was crossed with susceptible indica rice cultivar (BPT 5204) to generate the mapping population. A mendelian segregation ratio of 3:1 for resistant to susceptible F2 plants using bulk segregation analysis confirmed the presence of a major gene pi-1(t) by simple sequence repeats marker RM224 to the highly virulent blast isolate DRR 001.

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Acknowledgments

The authors are grateful to the Department of Biotechnology (DBT), New Delhi for providing financial assistance.

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Correspondence to B. Aruna Kanthi.

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Prasad, M.S., Kanthi, B.A., Balachandran, S.M. et al. Molecular mapping of rice blast resistance gene Pi-1(t) in the elite indica variety Samba mahsuri. World J Microbiol Biotechnol 25, 1765–1769 (2009). https://doi.org/10.1007/s11274-009-0074-7

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Keywords

  • Rice blast
  • Blast resistance
  • Microsatellite markers
  • M. grisea
  • Marker-assisted selection