Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
- 132 Downloads
Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made between TSR system and plants of the same genotype. The extraradical fungal structures generated in vitro culture showed normal development. Straight runner hyphae branched into short simple branched absorbing structures and spores were initiated. AM symbiosis was confirmed by the presence of arbuscules and vesicles in cortical cells of the TSRs. The frequency of intraradical colonization in TSRs was higher than in plants grown in soil, whereas the intensity values of intraradical colonization in TSR cultures were similar to those in whole plants. These results show that TSR cultures were able to support the growth and characteristic development of G. intraradices.
KeywordsTransformed soybean roots Monoxenic culture Glomus intraradices
We wish to thank to UBA, CONICET and ANCYPT for financial support. The authors acknowledge to Dr. Josefina Albergina of the University of Buenos Aires and to Dr. Elizabeth Agostini of the University of Rio Cuarto for technical assistance.
- Bago A, Cano C, Toussaint JP, Smith S, Dickson S (2006) Interactions between the arbuscular mycorrhizal (AM) fungus Glomus intraradices and nontransformed tomato roots of either wild-type or AM-defective phenotypes in monoxenic cultures. Mycorrhiza 16:429–436. doi: 10.1007/s00572-006-0054-9 CrossRefGoogle Scholar
- Barker SJ, Tagu D (2000) The roles of auxins and cytokinins in mycorrhizal symbioses. J Plant Growth Regul 19:144–154Google Scholar
- Bethlenfalvay GJ (1992) Mycorrhizae and crop productivity. In: Bethlenfalvay GJ, Linderman RG (eds) Mycorrhizae in sustainable agriculture. American Society of Agronomy, Madison, pp 1–27Google Scholar
- Boisson-Dernier A, Chabaud M, Garcia F, Bécard G, Rosenberg C, Barker D (2001) Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations. Mol Plant Microbe Interact 14:693–700. doi: 10.1094/MPMI.2001.14.6.695 CrossRefGoogle Scholar
- Cano C, Bago B (2006) Competition and substrate colonization strategies of three polyxenically-grown arbuscular mycorrhizal fungi. Mycologia 97:1214–1227Google Scholar
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
- Hewitt EJ (1952) Sand water culture methods used in the study of plant nutrition. Commonwealth Agriculture Bureau. Technical Communication No. 22Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
- Secretaría de Agricultura, Ganadería, Pesca y Alimento (2008). Estimaciones Agrícolas Mensuales.In:http://www.sagpya.gov.ar/new/00/agricultura/otros/estimaciones/pdfmensual/noviembre_2008.pdf. Accessed 20 Jan 2009
- Tanaka N (1997) Strategy for the production of secondary metabolites by pRi-transformed regenerants. Plant Tissue Cult Biotech 3:128–137Google Scholar