Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis

  • Laura Fernández
  • Vanesa Silvani
  • Josefina Bompadre
  • Mariana Pérgola
  • Alicia Godeas
Short Communication

Abstract

Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made between TSR system and plants of the same genotype. The extraradical fungal structures generated in vitro culture showed normal development. Straight runner hyphae branched into short simple branched absorbing structures and spores were initiated. AM symbiosis was confirmed by the presence of arbuscules and vesicles in cortical cells of the TSRs. The frequency of intraradical colonization in TSRs was higher than in plants grown in soil, whereas the intensity values of intraradical colonization in TSR cultures were similar to those in whole plants. These results show that TSR cultures were able to support the growth and characteristic development of G. intraradices.

Keywords

Transformed soybean roots Monoxenic culture Glomus intraradices 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Laura Fernández
    • 1
  • Vanesa Silvani
    • 1
  • Josefina Bompadre
    • 1
  • Mariana Pérgola
    • 1
  • Alicia Godeas
    • 1
  1. 1.Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresBuenos AiresArgentina

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