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Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production

  • Sutthinan Khamna
  • Akira Yokota
  • Saisamorn Lumyong
Original Paper

Abstract

A total of 445 actinomycete isolates were obtained from 16 medicinal plant rhizosphere soils. Morphological and chemotaxonomic studies indicated that 89% of the isolates belonged to the genus Streptomyces, 11% were non-Streptomycetes: Actinomadura sp., Microbispora sp., Micromonospora sp., Nocardia sp, Nonomurea sp. and three isolates were unclassified. The highest number and diversity of actinomycetes were isolated from Curcuma mangga rhizosphere soil. Twenty-three Streptomyces isolates showed activity against at least one of the five phytopathogenic fungi: Alternaria brassicicola, Collectotrichum gloeosporioides, Fusarium oxysporum, Penicillium digitatum and Sclerotium rolfsii. Thirty-six actinomycete isolates showed abilities to produce indole-3-acetic acid (IAA) and 75 isolates produced siderophores on chrome azurol S (CAS) agar. Streptomyces CMU-PA101 and Streptomyces CMU-SK126 had high ability to produced antifungal compounds, IAA and siderophores.

Keywords

Actinomycetes Antagonistic Indole-3-acetic acid Siderophores Biocontrol 

Notes

Acknowledgments

This work was supported by The Royal Golden Jubilee Ph.D. Program (PHD/0153/2546). We are grateful to Dr. Eric H. C McKenzie (Landcare Research, Private Bag 92170, Auckland, New Zealand) for improving the English text.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Sutthinan Khamna
    • 1
  • Akira Yokota
    • 2
  • Saisamorn Lumyong
    • 1
  1. 1.Department of Biology, Faculty of ScienceChiang Mai UniversityChiang MaiThailand
  2. 2.Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan

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