Antifungal and antibacterial activities of indigenous Streptomyces isolates from saline farmlands: prescreening, ribotyping and metabolic diversity
- 585 Downloads
A culture collection of 110 indigenous Streptomyces strains originally isolated from saline farmlands (Punjab, Pakistan) using stringent methods was screened biologically and chemically to investigate their potential for the production of bioactive secondary metabolites. In a biological screening the crude extracts obtained from the culture broth of selected strains were analysed for their activity against a set of test organisms, including Gram-positive, Gram-negative bacteria, fungi and microalgae using the disk diffusion bioassay method. Additionally a cytotoxicity test was performed by means of the brine shrimp microwell cytotoxicity assay. In a chemical screening each of the crude extracts was analysed by TLC using various staining reagents and by HPLC-MS/MS measurements. The results depicted an impressive chemical diversity of crude extracts produced by these strains. The taxonomic status of the selected strains was confirmed by preliminary physiological testing and 16S rRNA gene sequencing.
KeywordsIndigenous Streptomyces spp Prescreening Ribotyping Metabolite diversity HPLC-MS/MS
We are thankful to Dr. H. Frauendorf for mass spectra, and F. Lissy and A. Kohl for technical assistance. A financial support of this work by a grant from Higher Education Commission (HEC) of Pakistan under IRSIP is gratefully acknowledged.
- Burkhardt K, Fiedler HP, Grabley S, Thiericke R, Zeeck A (1996) New cineromycins and muscacins obtained by metabolic pattern analysis of Streptomyces griseoviridis (FH-S 1832) I. Taxonomy, fermentation, isolation, and biological activity. J Antibiot 49:432–437Google Scholar
- Gerhardt P, Murry RGE, Wood WA, Kreig NR (1994) Methods for general and molecular bacteriology. ASM Washington, DCGoogle Scholar
- Grabley S, Thiericke R, Zeeck A (1999) The chemical screening approach. In: Grabley S, Thiericke R (eds) Drug discovery from nature. Springer–Verlag, Heidelberg, pp 124–148Google Scholar
- Kantz T, Bold HC (1969) Morphological and taxonomic investigation of Nostoc Anabena in culture. Physiological studies, University of Texas (publication No. 6924), Austin, Texas, USAGoogle Scholar
- Laatsch H (2000) Mikroorganismen als biologische Quelle neuer Wirkstoffe. In: Kayser O, Müller RH (eds) Pharmazeutische Biotechnologie. Wissenschaftliche Verlagsges, Stuttgart, Germany, pp 13–43Google Scholar
- Laatsch H (2007) AntiBase 2007, a data base for rapid dereplication and structure determination of microbial natural products. Wiley–VCH: Weinheim, Germany (and annual updates); see http://wwwuser.gwdgde/~ucoc/laatsch/AntiBase.htm
- Lechevalier HA, Williams ST, Sharpe ME, Holt JG (1989) The Actinomycetes: a practical guide to genetic identification of actinomycetes. In: Bergey’s manual of systematic bacteriology. pp 2344–3330Google Scholar
- Locci R (1989) Streptomycetes and related genera. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology. Williams & Wilkins, Baltimore, pp 2451–2493Google Scholar
- Omura S (1992) The search for bioactive compounds from microorganisms, 1st edn. Springer, Berlin, Heidelberg, New YorkGoogle Scholar
- Wendisch FK, Kutzner HJ (1991) The family Streptomycetaceae. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds), The prokaryotes, A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications. Springer–Verlag, Berlin/New York, pp 965–968Google Scholar
- Yun BS, Hidaka T, Furihata K, Seto H (1994) Microbial metabolites with tip A promoter inducing activity. J Antibiot 47:969–975Google Scholar