Fusarium solani, Tax-3, a new endophytic taxol-producing fungus from Taxus chinensis
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An endophytic fungus, Tax-3, was isolated from barks of Taxus chinensis grown in the Qinba mountains, China. The strain was classified into Fusarium solani based on the morphological characteristics and the molecular phylogenetics inferred from the nuclear ribosomal DNA ITS sequences with the sequence similarity values of 100%. High performance liquid chromatography (HPLC) assay showed the F. solani, Tax-3, produced taxol with a higher yield of 163.35 μg/L in the reformative potato dextrose liquid medium (d), revealing its potential applications for taxol production.
KeywordsEndophytic fungi Fusarium solani ITS sequence Phylogenetic analysis Taxol Taxus chinensis
We would like to thank Tajalli Keshavarz and anonymous reviewers for their critical reviews of the manuscript and acknowledge Dr. Bin Liu for critical reading of the manuscript. The work was supported jointly by projects from Shaanxi Educational Committee (08JZ21, 08JK244, 08JK248).
- Bashyal B, Li JY, Strobel G, Hess WM, Sidhu R (1999) Seimatoantlerium nepalense, an endophytic taxol producing coelomycete from Himalayan yew (Taxus wallachiana). Mycotaxon 72:33–42Google Scholar
- Cai L, Jeewon R, Hyde KD (2005) Phylogenetic evaluation and taxonomic revision of Schizothecium based on ribosomal DNA and protein coding genes. Fungal Divers 19:1–21Google Scholar
- Guo BH, Wang YC, Zhou XW, Hu K, Tan F, Miao ZQ et al (2006) An endophytic taxol-producing fungus BT2 isolated from Taxus chinensis var. mairei. Afr J Biotechnol 10:875–877Google Scholar
- Kim BJ, Gibson DM, Shuler ML (2006) Effect of the plant peptide regulator, phytosulfokine-alpha, on the growth and taxol production from Taxus sp. suspension cultures. Biotechnol Prog 95:8–14Google Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Zhou DP, Ping WX, Sun JQ, Zhou XH, Liu XL, Yang DZ et al (2001) Isolation of taxol-producing fungus. J Microbiol 21:18–19Google Scholar