World Journal of Microbiology and Biotechnology

, Volume 24, Issue 10, pp 2171–2178

A DNA vaccine candidate for B. anthracis immunization, pcDNA3.1+PA plasmid, induce Th1/Th2 mixed responses and protection in mice

  • Fatemeh Vahedi
  • Jennelle Kyd
  • Gholamreza Moazeni Jula
  • Monavvar Afzalaghaeei
  • Mahdi Kianizadeh
  • Mahmoud Mahmoudi
Original Paper

Abstract

The protective antigen (PA) of Bacillus anthracis (B. anthracis) is a potent immunogen and a candidate subunit vaccine. To address the question whether antibodies raised against PA following injection of pcDNA3.1+PA plasmid, encoding PA, can protect against virulent B. anthracis two different regimens of PA based vaccines (DNA and live spore) were used. The groups of BALB/c mice that received live spores of the Sterne strain, naked pcDNA3.1 and naked pcDNA3.1+PA were compared to control groups. All groups were injected three times with 30-day intervals. Two weeks after the last immunization, all mice were subjected to challenge with a pathogenic strain of B.anthracis (C2). Blood samples were taken before each injection and challenge. Evaluation of the sera by ELISA method showed that DNA immunization using pcDNA3.1+PA plasmid resulted in an antibody profile representative of a mixed Th1 and Th2 response, with a skewing to a Th1 response. The group which received the naked pcDNA3.1+PA had a survival rate of >80%. This challenge assay revealed that antibodies raised following DNA vaccination against PA can confer strong protection, and resistance against virulent species of B. anthracis.

Keywords

DNA vaccine Bacillus anthracis pcDNA3.1 

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Fatemeh Vahedi
    • 1
  • Jennelle Kyd
    • 2
  • Gholamreza Moazeni Jula
    • 3
  • Monavvar Afzalaghaeei
    • 4
  • Mahdi Kianizadeh
    • 1
  • Mahmoud Mahmoudi
    • 5
  1. 1.Biotechnology DepartmentRazi Vaccine & Serum Research InstituteMashhadIran
  2. 2.Capricornia Center of Mucosal ImmunologyCentral Queensland UniversityRock HamptonAustralia
  3. 3.Department of Aerobic Bacterial Vaccine ProductionRazi Vaccine & Serum Research InstituteKarajIran
  4. 4.Department of Social MedicineMashhad University of Medical ScienceMashhadIran
  5. 5.Immunology Research CenterMashhad University of Medical ScienceMashhadIran

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