The responses of Ri-TDNA-transformed roots and arbuscular mycorrhizal fungi established on Ri-TDNA-transformed roots to lead-amended media was investigated in vitro. At exposure to increasing concentrations of lead (2–10 mg/l[ppm]), three Ri-TDNA-transformed root clones viz., Swa, Swb and Swc, exhibited profuse growth. At exposure to increasing concentrations of lead (0.1–5 ppm), the dual cultures of Ri-TDNA-transformed roots and arbuscular mycorrhizal fungi., Glomus lamellosum/Swa, Glomus intraradices/Swb and Glomus proliferum/Swc, exhibited tolerance to 5 ppm of lead. When subjected to one physiological stress, either exposure to Pb or inoculation with AM fungi, Ri-TDNA-transformed root clones exuded more phenols in the growth medium than retained in the roots. When subjected to dual physiological stress, mycorrhizal Ri-TDNA-transformed roots growing on Pb-enriched medium, the total phenol content increased in the roots and exudation into the medium decreased.
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The first author, Dr. Sharda W. Khade would like to thank Department of Biotechnology, Govt. of India for the award of fellowship to carry out Post Doctoral Work at TERI, New Delhi
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Khade, S.W., Adholeya, A. Effects of heavy metal (Pb) on arbuscular mycorrhizal fungi in vitro. World J Microbiol Biotechnol 24, 1663–1668 (2008). https://doi.org/10.1007/s11274-008-9681-y
- AM fungi
- In vitro
- Physiological stress
- Ri-TDNA-transformed roots