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World Journal of Microbiology and Biotechnology

, Volume 24, Issue 8, pp 1445–1450 | Cite as

Effect of some essential oils on mycelial growth of Penicillium digitatum Sacc.

  • Mahdi Yahyazadeh
  • Reza OmidbaigiEmail author
  • Rasoul Zare
  • Hossein Taheri
Original Paper

Abstract

The antifungal action of four essential oils of Foeniculum vulgare (fennel), Thymus vulgaris (thyme), Eugenia caryophyllata (Clove) and Salvia officinalis (sage) was tested in vitro against Penicillium digitatum Sacc. Direct contact and vapour phase were used to test the antifungal activity of these essential oils against P. digitatum that is responsible for green mould rot of citrus fruits. The vapour phase and direct contact of clove and thyme essential oils exhibited the strongest toxicity and totally inhibited the mycelial growth of the test fungus. Thyme and clove essential oils completely inhibited P. digitatum growth either when added into the medium 600 μl l−1 or by their volatiles with 24 μl per 8 cm diameter Petri dish. In in vitro mycelial growth assay showed fungistatic and fungicidal activity by clove and thyme essential oils. Sage and fennel oils did not show any inhibitory activity on this fungus. Scanning electron microscopy (SEM) was done to study the mode of action of clove oil in P. digitatum and it was observed that treatment with the oil leads to large alterations in hyphal morphology.

Keywords

Antifungal activity Chemical analysis Essential oils Penicillium digitatum Scanning electron microscopy 

Abbreviations

OsO4

Osmium tetroxide

PDA

Potato dextrose agar

PBS

Phosphate-buffer saline (pH 7.4)

EO

Essential oil

GC

Gas chromatography

SEM

Scanning electron microscopy

GC-MS

Gas chromatography combined with mass spectrometry

ANOVA

Analysis of variance

LSD

Least significant difference

RI

Retention indices

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Mahdi Yahyazadeh
    • 1
  • Reza Omidbaigi
    • 1
    Email author
  • Rasoul Zare
    • 2
  • Hossein Taheri
    • 3
  1. 1.Department of Horticulture, College of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Department of BotanyIranian Research Institute of Plant ProtectionTehranIran
  3. 3.Citrus Research InstituteRamsarIran

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