Bacterial community diversity assessment in municipal solid waste compost amended soil using DGGE and ARISA fingerprinting methods
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Bacterial community structure and diversity of Tunisian agricultural soil treated with different amounts of municipal solid waste compost (MSWC) and other fertilizers were studied using DGGE and ARISA fingerprinting methods. Sequence analysis of dominant DGGE bands revealed the presence of three major clusters, Cytophaga/Flexibacter/Bacteroides (CFB) group, Proteobacteria and Acidobacteria group. Using ARISA profiles, dominant populations were assigned to low and high GC Gram positive bacteria, Cyanobacteria, Spirochetes and Cytophagales. The two methods revealed the absence of significant bacterial community shifts related to the different MSWC applications. Moreover, indigenous bacterial population of the used loam-clayey soil was observed to limit proliferation and survival of Proteobacteria, initially dominant in MSWC and farmyard manure. Effectiveness of the two methods for soil bacterial community studying was shown. While DGGE was more accurate for bacterial identification, ARISA was more practical for handling and rapid estimation of dominant bacteria.
KeywordsARISA Bacterial community structure Compost DGGE
The authors would like to thank Ameur Cherif for his scientific advices. The present study is a part of the 1999–2006 research program “Municipal solid waste treatment and compost agricultural re-use” which is supported by the Tunisian State Secretariat of Scientific Research and Technology.
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