Characterization of a cold-tolerant plant growth-promoting bacterium Pantoea dispersa 1A isolated from a sub-alpine soil in the North Western Indian Himalayas

  • G. SelvakumarEmail author
  • S. Kundu
  • Piyush Joshi
  • Sehar Nazim
  • A. D. Gupta
  • P. K. Mishra
  • H. S. Gupta
Original paper


Pantoea dispersa strain 1A is a Gram-negative rod-shaped, yellow-pigmented bacterium isolated on nutrient agar plates incubated at 4°C. The identity of the bacterium was confirmed by sequencing of the 16 S rRNA gene. It was capable of growing at temperatures ranging from 4 to 42°C, but maximum growth was observed at 30°C. It is endowed with multiple plant growth promotion attributes such as phosphate solubilization, IAA production, siderophore production and HCN production, which are expressed differentially at sub-optimal temperatures (15 and 4°C). It was able to solubilize phosphate (17.6 μg of P2O5 ml−1 day−1), and produce IAA (3.7 μg ml−1 day−1), at 15°C. Qualitative detection of siderophore production and HCN were also observed at 15°C. At 4°C it was found to express all the plant growth promotion attributes. This bacterial isolate was able to positively influence and promote the growth and nutrient uptake parameters of wheat (cv. VL.802) under glasshouse conditions. Hence in the context, of cold wheat-growing environments, it is proposed that Pantoea dispersa 1A (MTCC 8706), could be deployed as an inoculant to attain the desired results of bacterization.


Cold tolerance Nutrient uptake Pantoea dispersa Plant growth promotion 



This study was supported by ICAR network project “Application of Microorganisms in Agriculture and Allied Sectors”.


  1. Ahmed S (1995) Agriculture–fertilization interface in Asia issue of growth and sustainability. Oxford and IBH publishing Co, New DelhiGoogle Scholar
  2. Astron B (1991) Role of bacterial cyanide production in differential reaction of plant cultivars to deleterious rhizosphere pseudomonads. Plant Soil 133:93–100CrossRefGoogle Scholar
  3. Atlas RM (1995) The handbook of microbiological media for the examination of food. Boca Raton, CRC Press, pp 197Google Scholar
  4. Bakker AW, Schipper B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biol Biochem 19:451–457CrossRefGoogle Scholar
  5. Cakmakc R, Kantar F, Sahin F (2001) Effect of N2-fixing bacterial inoculations on yield of sugar beet and barley. J Plant Nutr Soil Sci 164:527–531CrossRefGoogle Scholar
  6. Collins CH, Lyne PM (1980) Microbiological methods. Butterworth and Co. (Publishers) Ltd., LondonGoogle Scholar
  7. Elliot LF, Lynch JM (1984) Pseudomonas as a factor in the growth of winter wheat (Triticum aestivum L.). Soil Biol Biochem 16:69–71CrossRefGoogle Scholar
  8. Gavini F, Mergaert J, Beji A et al (1989) Transfer of Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov. Int J Sys Bacteriol 39:337–345CrossRefGoogle Scholar
  9. Gordon AS, Weber RP (1951) Colorimetric estimation of indole acetic acid. Plant Physiol 26:192–195CrossRefGoogle Scholar
  10. Greenland D, Losleben M (2001) Structure and function of an alpine ecosystem. In: Bowman WD, Seastedt TR (eds) Climate. Oxford University Press, Niwot Ridge, Colorado, New York, pp 15–31Google Scholar
  11. Hameeda B, Rupela OP, Reddy G (2006) Application of plant growth-promoting bacteria associated with composts and macrofauna for growth promotion of Pearl millet (Pennisetum glaucum L.). Bio Fertil Soils 43:221–227CrossRefGoogle Scholar
  12. Jackson ML (1973) Soil chemical analysis. Prentice Hall of India Pvt Ltd., New Delhi, pp 25–214Google Scholar
  13. Jones DL, Darrah PR (1994) Role of root derived organic acids in the mobilization of nutrients from the rhizosphere. Plant Soil 166:247–257CrossRefGoogle Scholar
  14. Khalid A, Arshad M, Zahir ZA (2004) Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. J Appl Microbiol 96:473–480CrossRefGoogle Scholar
  15. Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120CrossRefGoogle Scholar
  16. Kottmeier ST, Sullivan CW (1990) Bacterial biomass and production in pack ice of Antarctica marginal ice age zones. Deep Sea Res 37:1311–1330CrossRefGoogle Scholar
  17. Kremer RJ, Souissi T (2001) Cyanide production by rhizobacteria and potential for suppression of weed seedling growth. Curr Microbiol 43:182–186CrossRefGoogle Scholar
  18. Kumar S, Tamura K, Nei M (2004) MEGA3: Integrated software for Molecular Evolutionary Genetic Analysis and sequence alignment. Brief Bioinform 5:150–163CrossRefGoogle Scholar
  19. Lifshitz R, Kloepper JW, Kozlowski M et al (1987) Growth promotion of canola (rape-seed) seedlings by a strain of Peudomonas putida under gnotobiotic conditions. Can J Microbiol 8:102–106Google Scholar
  20. Lambers H (1982) Cyanide–resistant respiration: a non phosphorylating electron transport pathway act as an energy overflow. Physiol Plant 55:478–485CrossRefGoogle Scholar
  21. Mehta S, Nautiyal CS (2001) An efficient method for qualitative screening of phosphate-solubilizing bacteria. Curr Microbiol 43:51–56CrossRefGoogle Scholar
  22. Meyer AF, Lipson A, Martin AP et al (2004) Molecular and metabolic characterization of cold-tolerant alpine soil Pseudomonas Sensu stricto. App Env Microbiol 70(1):483–489CrossRefGoogle Scholar
  23. Murphy JP, Riley JP (1962) A modified single solution method for the determination of the phosphate in natural waters. Anal Chem Acta 27:31–36CrossRefGoogle Scholar
  24. Olsen SR, Sommers LE (1982) Phosphorus. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, part2. Chemical and microbiological properties. American Society of Agronomy. Madison, Wisconsin, USA, pp 403–430Google Scholar
  25. Pandey A, Palni LMS, Mulkalwar P et al (2002) Effect of temperature on solubilization of tricalcium phosphate by Pseudomonas corrugata. J Sci Indus Res 61:457–460Google Scholar
  26. Pandey A, Palni LMS (1998) Isolation of Pseudomonas corrugata from Sikkim Himalayas. World J Microbiol Biotechnol 14:411–413CrossRefGoogle Scholar
  27. Pandey A, Trivedi P, Kumar B et al (2006) Characterization of a phosphate solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a sub-alpine location in the Indian Central Himalaya. Curr Microbiol 53:102–107CrossRefGoogle Scholar
  28. Pikovaskaya RI (1948) Mobilization of the phosphorous in soil in connection with the vital activity of some microbial sp. Mikrobiologiya 17:362–370Google Scholar
  29. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
  30. Sarniguet A, Lucas P, Lucas M et al (1992) Soil conduciveness to take all of wheat: Influence of the nitrogen fertilizers on the structure of populations of fluorescent pseudomonads. Plant Soil 145:29–36CrossRefGoogle Scholar
  31. Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophore. Anal Biochem 160:47–56CrossRefGoogle Scholar
  32. Shivaji S, Chaturvedi P, Reddy GSN et al (2005) Pedobacter himalayensis sp. nov., from Hamta glacier located in the Himalayan mountain range in India. Int J Syst Evol Microbiol 55:1083–1088CrossRefGoogle Scholar
  33. Thompson JD, Gibson TJ, Plewniak F et al (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882CrossRefGoogle Scholar
  34. Zhang L, Birch RG (1997) Mechanisms of biocontrol by Pantoea dispersa of sugar cane leaf scald disease caused by Xanthomonas albilineans. J App Microbiol 82:448–454CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • G. Selvakumar
    • 1
    Email author
  • S. Kundu
    • 1
  • Piyush Joshi
    • 1
  • Sehar Nazim
    • 1
  • A. D. Gupta
    • 1
  • P. K. Mishra
    • 1
  • H. S. Gupta
    • 1
  1. 1.Vivekananda Institute of Hill AgricultureIndian Council of Agricultural ResearchAlmoraIndia

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