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Lactate increases coenzyme Q10 production by Agrobacterium tumefaciens

  • Suk-Jin Ha
  • Sang-Yong Kim
  • Jin-Ho Seo
  • Won-Il Sim
  • Hee-Jung Moon
  • Jung-Kul LeeEmail author
Original Paper

Abstract

By the optimization of nitrogen source for coenzyme Q10 (ubiquinone, CoQ10) production in Agrobacterium tumefaciens KCCM 10413 culture, the highest CoQ10 production was achieved in medium containing corn steep powder (CSP). Components for a stimulatory effect on the production of CoQ10 in CSP were screened, and lactate was found to increase dry cell weight (DCW) and the specific CoQ10 content. In a fed-batch culture of A. tumefaciens, supplementation with 1.5 g of lactate l−1 further improved DCW, the specific CoQ10 content, and CoQ10 production by 16.0, 5.8, and 22.8%, respectively. It has been reported that lactate stimulates cell growth and acts as an accelerator driving the tricarboxylic acid (TCA) cycle (Roberto et al. 2002, Biotechnol Let 24:427–431; Matsuoka et al. 1996, Biosci Biotechnol Biochem 60:575–579). In this study, lactate supplementation increased DCW and the specific CoQ10 content in A. tumefaciens culture, probably by accelerating TCA cycle and energy production as reported previously, leading to the increase of CoQ10 production.

Keywords

Coenzyme Q10 Agrobacterium tumefaciens CSP Lactate ATP Ubiquinone 

Notes

Acknowledgment

This paper was supported by Konkuk University in 2007.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Suk-Jin Ha
    • 1
    • 2
  • Sang-Yong Kim
    • 1
  • Jin-Ho Seo
    • 2
  • Won-Il Sim
    • 3
  • Hee-Jung Moon
    • 4
  • Jung-Kul Lee
    • 3
    Email author
  1. 1.BioNgene Co., LtdSeoulKorea
  2. 2.Department of Bioscience and BiotechnologySeoul National UniversitySeoulKorea
  3. 3.Department of Chemical EngineeringKonkuk UniversitySeoulKorea
  4. 4.Department of Bioscience and BiotechnologyKonkuk UniversitySeoulKorea

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