A novel fibrinolytic enzyme from Cordyceps militaris, a Chinese traditional medicinal mushroom

  • Li Cui
  • Ming Sheng DongEmail author
  • Xiao Hong Chen
  • Mei Jiang
  • Xin Lv
  • Guijun Yan
Original Paper


A novel fibrinolytic enzyme from Cordyceps militaris was purified and partially characterized for the first time, which was designated C. militaris fibrinolytic enzyme (CMase). This extracellular enzyme from C. militaris was isolated by ammonium sulphate fraction, and purified to electrophoretic homogeneity using gel filtration chromatography. The apparent molecular mass of the purified enzyme was estimated to be 27.3 kDa by SDS-PAGE. The optimum pH and temperature for the enzyme activity were pH 6.0 and 25 °C, respectively. In the presence of metal ions such as Mg2+ and Fe2+ ions the activity of the enzyme increased, whereas EDTA and Cu2+ ion inhibited the enzyme activity. Interestingly the N-terminal amino acid sequences of the enzyme is extremely similar to those of the trypsin proteinases from insects, and has no significant homology with those of the fibrinolytic enzyme from other medicinal mushroom. In conclusion, C. militaris produces a strong fibrinolytic enzyme CMase and may be considered as a new source for thrombolytic agents.


Cordyceps militaris CMase Entomogenous fungus Fibrinolytic activity Medicinal mushroom 



Authors are grateful to Dr. Huaan Yang, Agriculture Department of Western Australia, Australia, for careful and critical reading of the manuscript. This work was supported by High-Tech Research and Development Program of China (No: 2006AA10Z343) and Key Scientific Project of Jiangsu Province, China (No: BE2006325).


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Li Cui
    • 1
  • Ming Sheng Dong
    • 1
    Email author
  • Xiao Hong Chen
    • 1
  • Mei Jiang
    • 1
  • Xin Lv
    • 1
  • Guijun Yan
    • 2
  1. 1.College of Food Science and Technology Nanjing Agricultural UniversityNanjingP.R. China
  2. 2.School of Plant Biology, Faculty of Natural and Agricultural Sciences The University of Western AustraliaCrawleyAustralia

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