Arbuscular mycorrhizal (AM) technology for the conservation of Curculigo orchioides Gaertn.: an endangered medicinal herb

  • Deepika Sharma
  • Rupam Kapoor
  • Ashok K. Bhatnagar
Original Paper

Abstract

Curculigo orchioides Gaertn. (family Hypoxidaceae) is an endangered anticarcinogenic and aphrodisiac herb, native of India. This study reports the effect of three arbuscular mycorrhizal (AM) fungal inocula on post-transplanting performance of ‘in vitro’ raised C. orchioides plantlets. The three AM fungal inocula consisted of two monospecific cultures of Glomus geosporum and G. microcarpum and one crude consortium of AM fungal spores isolated from rhizosphere soil of C. orchioides growing in natural habitat. Complete plantlets of C. orchioides were raised by direct organogenesis of leaf explants on half strength Murashige and Skoog’s medium devoid of any growth hormone. C. orchioides plantlets responded significantly different to all three mycorrhizal treatments. Mycorrhization enhanced the survival rate of C. orchioides plantlets to 100%. The inoculated plantlets fared significantly better than the uninoculated ones in terms of biomass production and number of leaves and roots per plant. Mycorrhizal plantlets exhibited higher concentrations of photosynthetic pigments as well as minerals P, Mg, Cu, Zn, Mn and Fe in both shoots and roots. Among the three inocula tested, plantlets inoculated with the mixed consortium of AM fungi consistently performed better in terms of the parameters evaluated. The study suggests use of mixed consortium of AM fungi over monospecific cultures for the sustainable cultivation and conservation of endangered medicinal plant: Curculigo orchioides.

Keywords

Glomus geosporum Glomus microcarpum Medicinal plant Micropropagation Mixed consortium 

Abbreviations

AM

Arbuscular mycorrhiza

GG

Glomus geosporum

GM

Glomus microcarpum

MC

Mixed consortium

Notes

Acknowledgements

This research was funded by Department of Science and Technology, New Delhi, India. The authors are thankful to Prof. S. R. Yadav for helping in collection of the plant material.

References

  1. Allen SE (1989) Chemical analysis of ecological materials. 2nd edn. Blackwell Scientific Publishers, UKGoogle Scholar
  2. Arnon DJ (1949) Copper enzyme in isolated chloroplasts polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15CrossRefGoogle Scholar
  3. Bafna AR, Mishra SH (2005) Actividad antioxidante in vitro del extracto de methanol de los rizomas de Curculigo orchioides Gaertn. Ars Pharm 46(2):125–138Google Scholar
  4. Brundrett M, Juniper S (1995) Non-destructive assessment of spore germination of VAM fungi and production of pot cultures from single spores. Soil Biol Biochem 27:85–91CrossRefGoogle Scholar
  5. Clapperton MJ, Reid DM (1992) A relationship between plant growth and increasing VA mycorrhizal inoculum density. New Phytol 120:227–234CrossRefGoogle Scholar
  6. Estrada-Luna AA, Davies FT (2003) Arbuscular mycorrhizal fungi influence water relations, gas exchange, abscissic acid and growth of micropropagated chile ancho pepper (Capsicum annum) plantlets during acclimatization and post-acclimatization. J Plant Physiol 160:1073–1083CrossRefGoogle Scholar
  7. Estrada-Luna AA, Davies FT Jr, Egilla JN (2000) Mycorrhizal fungi enhancement of growth and gas exchange of micropropagated guava (Psidium guajava L.) during ex vitro acclimatization and plant establishment. Mycorrhiza 10:1–8CrossRefGoogle Scholar
  8. Gaur A, Adholeya A (1999) Mycorrhizal effects on the acclimatization, survival, growth and chlorophyll of micropropagated Syngonium and Draceana inoculated at weaning and hardening stages. Mycorrhiza 9:215–219CrossRefGoogle Scholar
  9. Gerdemann JW, Nicolson TH (1963) Spores of mycorrhizal species extracted from soil by wet sieving and decanting method. Trans Brit Mycol Soc 46:235–244CrossRefGoogle Scholar
  10. Giri B, Kapoor R, Mukerji KG (2007) Improved tolerance of Acacia nilotica to salt stress by arbuscular mycorrhiza, Glomus fasciculatum is partly related to elevated K: Na ratios in root and shoot tissues. Micro Ecol (in press)Google Scholar
  11. Giri B, Kapoor R, Mukerji KG (2005) Effect of arbuscular mycorrhiza Glomus fasciculatum and G. macrocarpum on the growth and nutrient content of Cassia siamea in semi-arid Indian wasteland soil. New Forest 29:63–73CrossRefGoogle Scholar
  12. Grout BWW, Millan S (1985) Photosynthetic development of micropropagated strawberry plantlets following transplanting. Ann Bot 55:129–131Google Scholar
  13. Hiscox JD, Israelstam CP (1979) A method for extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1331–1334CrossRefGoogle Scholar
  14. Jaizme-Vega MC, Rodriguez-Romero AS, Hermoso CM et al (2003) Growth of micropropagated bananas colonized by root-organ culture produced arbuscular mycorrhizal fungi entrapped in Ca-alginate beads. Plant Soil 254:329–335CrossRefGoogle Scholar
  15. Kapoor R, Bhatnagar AK (2007) Attenuation of cadmium toxicity in mycorrhizal Celery (Apium graveolens L.). World J Microbiol Biotechnol DOI 10.1007/s11274-006-9337-8Google Scholar
  16. Kapoor R, Giri B, Mukerji KG (2002) Glomus macrocarpum: a potential bioinoculant to improve essential oil quality and concentration in dill (Anethum graveolens L.) and carum (Trachyspermum ammi (Linn.) Sprague). World J Microbiol Biotechnol 18:459–463CrossRefGoogle Scholar
  17. Kapoor R, Giri B, Mukerji KG (2004) Improved growth and essential oil yield and quality in Foeniculum vulgare Mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresource Technol 93:309–311CrossRefGoogle Scholar
  18. Munkrold L, Kjoller R, Vestberg M et al (2004) High functional diversity within species of arbuscular mycorrhizal fungi. New Phytol 164:357–364CrossRefGoogle Scholar
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  20. Ning J, Cumming JR (2001) Arbuscular mycorrhizal fungi alter phosphorus relations of broomsedge (Andropogon virginicus L.) plants. J Exp Bot 52:1883–1891CrossRefGoogle Scholar
  21. Parke JL, Kaeppler SW (2000) Effects of genetic differences among crop species and cultivars upon the arbuscular mycorrhizal symbiosis. In: Kapulnik K, Douds DD (eds) Arbuscular mycorrhizas: physiology and function. Kluwer, The Netherlands, pp 131–146Google Scholar
  22. Prajapati HA, Patel DH, Mehta SP et al (2003) Direct in vitro regeneration of Curculigo orchioides Gaertn., an endangered anticarcinogenic herb. Curr Sci 84:747–749Google Scholar
  23. Rai MK (2001) Current advances in mycorrhization in micropropagation. In Vitro Cell Dev Biol-Plant 37:158–167CrossRefGoogle Scholar
  24. Ravnskov S, Jakobsen I (1995) Functional compatibility in arbuscular mycorrhizas measured as hyphae P transport to the plant. New Phytol 129:611–618CrossRefGoogle Scholar
  25. Schenck NC, Perez Y (1990) Manual for the Identification of VA Mycorrhizal fungiGoogle Scholar
  26. Smith FA, Jakobsen I, Smith SE (2000) Spatial differences in acquisition of soil phosphate between two arbuscular mycorrhizal fungi in symbiosis with Medicago truncatula. New Phytol 147:357–366CrossRefGoogle Scholar
  27. Sylvia DM, Williams SE (1992) Vesicular-arbuscular mycorrhizae and environmental stress. In: Bethlenfalvay GJ, Linderman RG (eds) Mycorrhizae in sustainable agriculture. Special Publication 54, ASA, Madison, Wisc, pp 101–124Google Scholar
  28. Taiz L, Zeiger E (1998) Plant Physiology. Second ed., Sinauer Assoc. Inc. Pub., USAGoogle Scholar
  29. Vaidya GK, D’Cruz L, Saxena OP et al (2005) Effect of various factors on plantlet regeneration of Curculigo orchioides Gaertn. Plant Cell Biotech Mol Biol 6:29–34Google Scholar
  30. van der Heijen MGA, Klironomos JN, Moutoglis P et al (1998) Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396:69–72CrossRefGoogle Scholar
  31. Wala BB, Jasrai YT (2003) Micropropagtion of an endangered medicinal plant: Curculigo orchioides Gaertn. Plant Tissue Cult 13:13–19Google Scholar
  32. Wang H, Parent S, Gosselin A et al (1993) Study of vesicular-arbuscular mycorrhizal peat-based substrates on symbiosis establishment, acclimatization and growth of three micropropagated species. J Am Soc Hort Sci 118:896–901Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Deepika Sharma
    • 1
  • Rupam Kapoor
    • 1
  • Ashok K. Bhatnagar
    • 1
  1. 1.Environmental Biology Laboratory, Department of BotanyUniversity of DelhiDelhiIndia

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