Phosphate solubilization by Penicillium spp. isolated from soil samples of Indian Himalayan region

  • Anita PandeyEmail author
  • Namrata Das
  • Bhavesh Kumar
  • K. Rinu
  • Pankaj Trivedi
Original Paper


A total of 246 fungal isolates representing 36 genera and 72 species were isolated from the soil samples collected from Indian Himalayan region. Twenty-one species belonged to the genus Penicillium alone. All the Penicillium species were screened for phosphate solubilizing activity on Pikovskaya agar at 21 °C. Eight species of Penicillium, exhibiting formation of halos (zone of solubilization) around the fungal colonies in qualitative plate assays, were selected for quantitative estimations. In quantitative estimations that were conducted upto day 30 (at 3 days interval), seven species of Penicillium brought maximum solubilization after day 15, while P. oxalicum showed maximum solubilization after day 21 of incubation. The increase in solubilization coincided with decrease in pH of the broth. Acid phosphatase activity was 1.5–2.0 times higher in comparison to alkaline phosphatase. Many of these species showed wide range of tolerance for temperature, pH and salt concentration.


Penicillium Phosphate-solubilization Phosphatase Himalaya 



Dr. LMS Palni is acknowledged for his interest in this research. Director, GBPIHED, is acknowledged for providing the facilities. The Department of Biotechnology and the Ministry of Environment and Forests, Govt. of India, New Delhi, are thanked for financial support.


  1. Asea PEA, Kucey RMN, Stewart JWB (1988) Inorganic phosphate solubilization by two Penicillium species in solution culture and soil. Soil Biol Biochem 20:450–464CrossRefGoogle Scholar
  2. Baath E (1981) Microfungi in a clear-cut pine forest soil in Central Sweden. Can J Bot 59(7):1331–1337CrossRefGoogle Scholar
  3. Bagyaraj DJ, Krishnaraj PU, Khanuja SPS (2000) Mineral phosphate solubilization: agronomic implications, mechanism and molecular genetics. Proc Indian Natl Sci Acad (PINSA) B66(2&3):69–82Google Scholar
  4. Banik S, Dey BK (1982) Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate solubilizing microorganisms. Plant Soil 69:353–364CrossRefGoogle Scholar
  5. Chen X, Tang J, Fang Z, Shuijin HU (2002) Phosphate-solubilizing microbes in rhizosphere soils of 19 weeds in southeastern China. J Zhejiang Univ Sci 3(3):355–361CrossRefGoogle Scholar
  6. Cunningham JE, Kuiack C (1992) Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium bilaii. Appl Environ Microbiol 58:1451–1458Google Scholar
  7. Illmer P, Schinner F (1992) Solubilization of inorganic phosphates by microorganisms isolated from forest soils. Soil Biol Biochem 27:260–270Google Scholar
  8. Kucey RMN, Leggett ME (1989) Increased yields and phosphorus uptake by Westar canola (Brassica napus L.) inoculated with a phosphate-solubilizing isolate of Penicillium bilaji. Can J Soil Sci 69:425–432CrossRefGoogle Scholar
  9. Larena I, Melgarejo P, de Cal A (2002) Production, survival, and evaluation of solid-substrate inocula of Penicillium oxalicum, a biocontrol agent against Fusarium wilt of tomato. Phytopathology 92(8):863–869CrossRefGoogle Scholar
  10. Nahas E (1996) Factors determining rock phosphate solubilization by microorganisms isolated from soil. World J Microbiol Biotechnol 12:567–572CrossRefGoogle Scholar
  11. Nautiyal CS, Bhaduria S, Kumar P, Lal H, Mondal R, Verma D (2000) Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiol Lett 182:291–296CrossRefGoogle Scholar
  12. Omar SA (1998) The role of rock-phosphate-solubilizing fungi and vesicular-arbuscular-mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate. World J Microbiol Biotechnol 14(2):211–218CrossRefGoogle Scholar
  13. Pandey A, Trivedi P, Kumar B, Chaurasia B, Singh S, Palni LMS (2004) Development of microbial inoculants for enhancing plant performance in mountains. In: Reddy MS, Khanna S (eds) Biotechnological approaches for sustainable development. Allied Publishers Pvt. Limited, New DelhiGoogle Scholar
  14. Pandey A, Palni LMS, Mulkalwar P, Nadeem M (2002) Effect of temperature on solubilization of tricalcium phosphate by Pseudomonas corrugata. J Sci Ind Res 61:457–460Google Scholar
  15. Pandey A, Trivedi P, Kumar B, Palni LMS (2006) Characterization of a phosphate solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a Sub-Alpine location in the Indian Central Himalaya. Curr Microbiol 53:102–107CrossRefGoogle Scholar
  16. Reyes I, Bernier L, Antoun H (2002) Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum. Microb Ecol 44(1):39–48CrossRefGoogle Scholar
  17. Schachtman DP, Reid RJ, Ayling SM (1998) Phosphate uptake by plants from soil to cell. Plant Physiol 116:447–453CrossRefGoogle Scholar
  18. Tabatabai MA, Bremner JM (1969) Use of p-nitrophenol phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307CrossRefGoogle Scholar
  19. Tarafdar JC, Bareja M, Panwar J (2003) Efficiency of some phosphatase producing soil-fungi. Indian J Microbiol 43:27–32Google Scholar
  20. Vassilev N, Fenice M, Federici F (1996) Rock phosphate solubilization with gluconic acid produced by immobilized Penicillium variabile P16. Biotechnol Tech 10:585–588CrossRefGoogle Scholar
  21. Vazquez P, Holguin G, Puente ME, Lopez-Cortes A, Bashan Y (2000) Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biol Fertil Soils 30:460–468CrossRefGoogle Scholar
  22. Wakelin SA, Warren RA, Harvey PR, Ryder MH (2004) Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biol Fertil Soils 40:36–43CrossRefGoogle Scholar
  23. Wahid OA, Mehana TA (2000) Impact of phosphate-solubilizing fungi on the yield and phosphorus-uptake by wheat and faba bean plants. Microbiol Res 155:221–227Google Scholar
  24. Whitelaw MA (2000) Growth promotion of plants inoculated with phosphate solubilizing fungi. Adv Agron 69:99–151CrossRefGoogle Scholar
  25. Widden P (1987) Fungal communities in soils along an elevational gradient in Northern England. Mycologia 79:298–309CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Anita Pandey
    • 1
    Email author
  • Namrata Das
    • 1
  • Bhavesh Kumar
    • 1
  • K. Rinu
    • 1
  • Pankaj Trivedi
    • 1
  1. 1.Environmental Physiology and BiotechnologyGB Pant Institute of Himalayan Environment and DevelopmentAlmoraIndia

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