World Journal of Microbiology and Biotechnology

, Volume 24, Issue 1, pp 79–87

Optimization of process parameters for improved production of bioactive metabolite by a novel endophytic fungus Fusarium sp. DF2 isolated from Taxus wallichiana of North East India

  • Dip K. Gogoi
  • Hari P. Deka Boruah
  • Ratul Saikia
  • Tarun C. Bora
Original Paper

DOI: 10.1007/s11274-007-9442-3

Cite this article as:
Gogoi, D.K., Deka Boruah, H.P., Saikia, R. et al. World J Microbiol Biotechnol (2008) 24: 79. doi:10.1007/s11274-007-9442-3

Abstract

An endophytic fungus having antifungal and antibacterial properties was isolated from Taxus wallichiana of Arunachal Pradesh, India. On the basis of morphological and molecular characteristics, the fungus was identified as Fusarium sp. and designated as DF2. The fungus was optimized for growth and maximum production of the antimicrobial agent. Media with 5% leaf extract (w/v) supplemented with 0.1% dextrose as carbon and yeast extract as nitrogen source favored the growth with temperature optimum 25 ± 2°C and pH 6. Incubation period of 10 days was observed optimum for growth and production of antimicrobial agent. Phenylalanine and dextrose enriched basal medium promoted the antimicrobial agent production, whereas methionine amended in combination with glucose promoted higher biomass accumulation. The TLC purified active compound with UV λ-max 270 nm in ethyl acetate has got the lowest minimum inhibitory concentration (MIC) against Bacillus subtilis, Staphylococcus aureus and Escherichia coli and highest against Pseudomonas aeruginosa.

Keywords

Antimicrobial agent Endophytes Fusarium sp. MIC 

Abbreviations

PDA

Potato dextrose agar

NA

Nutrient agar

MHA

Muellar hinton agar

MTCC

Microbial type culture collection

ATCC

American type culture collection

IMTECH

Institute of microbial technology

ICRISAT

International crop research institute for the semi arid tropics

MIC

Minimum inhibitory concentration

MFC

Minimum fungicidal concentration

qp

Specific rate of product production

NCBI

National centre for biotechnology information

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Dip K. Gogoi
    • 1
  • Hari P. Deka Boruah
    • 1
  • Ratul Saikia
    • 1
  • Tarun C. Bora
    • 1
  1. 1.Biotechnology DivisionRegional Research Laboratory (CSIR)JorhatIndia

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