World Journal of Microbiology and Biotechnology

, Volume 23, Issue 12, pp 1725–1731 | Cite as

Enzymatic hydrolysis of soybean oil using lipase from different sources to yield concentrated of polyunsaturated fatty acids

  • Larissa Freitas
  • Tânia Bueno
  • Victor H. Perez
  • Júlio C. Santos
  • Heizir Ferreira de Castro
Original Paper


The ability of three commercially available lipases to mediate the hydrolysis of the soybean oil to yield concentrated of essential fatty acids was evaluated. The tested lipases were from microbial (Candida rugosa and Thermomyces lanuginosa) and animal cells (Porcine pancreatic lipase). In terms of free fatty acids, microbial lipases were more effective to promote the enzymatic hydrolysis of the soybean oil (over 70%) than the porcine pancreatic lipase (24%). In spite of this, porcine pancreatic lipase (PPL) showed the most satisfactory specificity towards both essential fatty acids and was, therefore, chosen to carry out additional studies. An experimental design was performed taking into consideration the enzyme and NaCl amounts as independent variables. The main effects were fitted by multiple regression analysis to a linear model and maximum fatty acids concentration could be obtained using 3.0 wt% of lipase and 0.08 wt% of NaCl. The mathematical model representing the hydrolysis degree was found to describe adequately the experimental results. Under these conditions, concentrations of 29.5 g/L and 4.6 g/L for linoleic and linolenic acids, respectively, were obtained.


Experimental design Hydrolysis Linoleic acid (omega-6) Linolenic acid (omega-3) Lipase Soybean oil 



Essential fatty acids


Polyunsaturated fatty acids


Fatty acid(s)


Fatty acid methyl esters



The authors acknowledge the financial assistance from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Larissa Freitas
    • 1
  • Tânia Bueno
    • 1
  • Victor H. Perez
    • 1
  • Júlio C. Santos
    • 1
  • Heizir Ferreira de Castro
    • 1
  1. 1.Engineering School of LorenaUniversity of São PauloLorenaBrazil

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