World Journal of Microbiology and Biotechnology

, Volume 23, Issue 12, pp 1653–1660 | Cite as

Phytate-degrading enzyme production by bacteria isolated from Malaysian soil

  • Anis Shobirin Meor Hussin
  • Abd-ElAziem Farouk
  • Ralf Greiner
  • Hamzah Mohd Salleh
  • Ahmad Faris Ismail
Original Paper
First Online:
Received:
Accepted:

Abstract

Over two hundred bacteria were isolated from the halosphere, rhizosphere and endophyte of Malaysian maize plantation and screened for phytases activity. Thirty isolates with high detectable phytase activity were chosen for media optimization study and species identification. Ten types of bacterial phytase producers have been discovered in this study, which provides opportunity for characterization of new phytase(s) and various commercial and environmental applications. The majority of the bacterial isolates with high detectable phytase activity were of endophyte origin and 1.6% of the total isolates showed phytase activity of more than 1 U/ml. Most of the strains produced extra-cellular phytase and Staphylococcus lentus ASUIA 279 showed the highest phytase activity of 1.913 U/ml. All 30 species used in media optimization study exhibit favorable enzyme production when 1% rice bran was included in the growth media.

Keywords

Screening phytase Zea mays Soil bacteria Media optimization 
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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Anis Shobirin Meor Hussin
    • 1
  • Abd-ElAziem Farouk
    • 1
  • Ralf Greiner
    • 2
  • Hamzah Mohd Salleh
    • 1
  • Ahmad Faris Ismail
    • 1
  1. 1.Biomolecular Engineering Research Unit, Department of Biotechnology EngineeringInternational Islamic University MalaysiaKuala LumpurMalaysia
  2. 2.Centre for Molecular BiologyFederal Research Centre for Nutrition and FoodKarlsruheGermany

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