World Journal of Microbiology and Biotechnology

, Volume 23, Issue 10, pp 1459–1464 | Cite as

Increased production of laccase by Cerrena unicolor in submerged liquid cultures

  • Grzegorz Janusz
  • Jerzy Rogalski
  • Janusz Szczodrak
Original Paper

Abstract

The wood-degrading basidiomycete Cerrena unicolor C-139 has been suggested as a potential producer of the industrially important enzyme laccase. Basic culture parameters influencing the enzyme synthesis in shaken-flask and aerated bioreactor cultures were evaluated to improve the yields of the process. Production of extracellular laccase was considerably enhanced by the addition of Cu2+ in the micromolar range to a carbon-sufficient and nitrogen-sufficient culture medium (C/N = 16.69). When an optimised medium containing glucose (10 g/L) and l-asparagine (1.5 g/L) was used, and enzyme synthesis was stimulated by addition of 10 μM Cu2+ to the culture medium on days 3, 6 and 9, maximal laccase productivity obtained after 17 days’ cultivation in shaken flask cultures was above 100,000 nkat/L. In fermenter fungal cultures, the influence of stabilisation of medium pH on laccase activity was additionally studied. The use of a bioreactor with an automatic pH control set at pH 6.5 after 48-h incubation resulted in the enzyme activity of 65,000 nkat/L after 8 days’ cultivation.

Keywords

Cerrena unicolor Laccase Culture factors Optimisation Increased productivity Submerged cultures 

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Grzegorz Janusz
    • 1
  • Jerzy Rogalski
    • 1
  • Janusz Szczodrak
    • 2
  1. 1.Department of BiochemistryMaria Curie-Sklodowska UniversityLublinPoland
  2. 2.Department of Industrial MicrobiologyMaria Curie-Sklodowska UniversityLublinPoland

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