World Journal of Microbiology and Biotechnology

, Volume 23, Issue 10, pp 1497–1501 | Cite as

Ethanol production from sweet sorghum juice in batch and fed-batch fermentations by Saccharomyces cerevisiae

  • Lakkana LaopaiboonEmail author
  • Pornthap Thanonkeo
  • Prasit Jaisil
  • Pattana Laopaiboon
Original Paper


Sweet sorghum juice supplemented with 0.5% ammonium sulphate was used as a substrate for ethanol production by Saccharomyces cerevisiae TISTR 5048. In batch fermentation, kinetic parameters for ethanol production depended on initial cell and sugar concentrations. The optimum initial cell and sugar concentrations in the batch fermentation were 1 × 108 cells ml−1 and 24 °Bx respectively. At these conditions, ethanol concentration produced (P), yield (Y ps) and productivity (Q p ) were 100 g l−1, 0.42 g g−1 and 1.67 g l−1 h−1 respectively. In fed-batch fermentation, the optimum substrate feeding strategy for ethanol production at the initial sugar concentration of 24 °Bx was one-time substrate feeding, where P, Y ps and Q p were 120 g l−1, 0.48 g g−1 and 1.11 g l−1 h−1 respectively. These findings suggest that fed-batch fermentation improves the efficiency of ethanol production in terms of ethanol concentration and product yield.


Batch fermentation Fed-batch fermentation Ethanol production Saccharomyces cerevisiae Sweet sorghum 



This research was financially supported by the National Research Council of Thailand (NRCT), Thailand. We would like to thank our students, Ms. Suparat Meekhun, Ms. Wannapa Glangsoom and Ms. Angkana Narongkasemsuk, for their technical assistance.


  1. Altıntaş MM, Ülgen KÖ, Kirdar B, Önsan ZI, Oliver SG (2002) Improvement of ethanol production from starch by recombinant yeast through manipulation of environmental factors. Enzyme Microb Technol 31:640–647CrossRefGoogle Scholar
  2. Amutha R, Gunasekaran P (2001) Production of ethanol from liquefied cassava starch using co-immobilized cells of Zymomonas mobilis and Saccharomyces diastaticus. J Biosci Bioeng 92:560–564CrossRefGoogle Scholar
  3. Anderson IC (2005) Ethanol from sweet sorghum. Cited 5 Oct 2005
  4. Bulawayo B, Bvochora JM, Muzondo MI, Zvauya R (1996) Ethanol production by fermentation of sweet stem sorghum juice using various yeast strains. World J Microbiol Biotechnol 12:357–360CrossRefGoogle Scholar
  5. Bvochora JM, Read JS, Zvauya R (2000) Application of very high gravity technology to the cofermentation of sweet stem sorghum juice and sorghum grain. Ind Crop Prod 11:11–17CrossRefGoogle Scholar
  6. Göksungur Y, Zorlu N (2001) Production of ethanol from beet molasses by Ca-alginate immobilized yeast cells in a packed-bed bioreactor. Turk J Biol 25:265–275Google Scholar
  7. Krishnan MS, Taylor F, Davison BH, Nghiem NP (2000) Economic analysis of fuel ethanol production from corn starch using fluidized-bed bioreactor. Biores Technol 75:99–105CrossRefGoogle Scholar
  8. Mecozzi M (2005) Estimation of total carbohydrate amount in environmental samples by the phenol-sulphuric acid method assisted by multivariate calibration. Chemom Intell Lab Syst 79:84–90CrossRefGoogle Scholar
  9. Ogbonna JC, Mashima H, Tanaka H (2001) Scale up of fuel ethanol production from sugar beet juice using loofa sponge immobilized bioreactor. Biores Technol 76:1–8CrossRefGoogle Scholar
  10. Rajvanshi AK, Nimbkar N (2005) Sweet sorghum R&D at the Nimbkar Agricultural Research Institute (NARI). Cited 5 Oct 2005Google Scholar
  11. Stanbury PF, Whitaker A, Hall SJ (1995) Principles of fermentation technology. Pergamon Press, OxfordGoogle Scholar
  12. Zhan X, Wang D, Tuinstra MR, Bean S, Seib PA, Sun XS (2003) Ethanol and lactic acid production as affected by sorghum genotype and location. Ind Crop Prod 18:245–255CrossRefGoogle Scholar
  13. Zoecklien BW, Fugelsang KC, Gump BH, Nury FS (1995) Laboratory procedures. In: Wine analysis and production. Chapman & Mall, New YorkGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Lakkana Laopaiboon
    • 1
    • 2
    Email author
  • Pornthap Thanonkeo
    • 1
  • Prasit Jaisil
    • 3
  • Pattana Laopaiboon
    • 1
  1. 1.Department of Biotechnology, Faculty of TechnologyKhon Kaen UniversityKhon KaenThailand
  2. 2.Fermentation Research Center for Value Added Agricultural ProductsKhon Kaen UniversityKhon KaenThailand
  3. 3.Department of Agronomy, Faculty of AgricultureKhon Kaen UniversityKhon KaenThailand

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