Ethanol production from sweet sorghum juice in batch and fed-batch fermentations by Saccharomyces cerevisiae
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Sweet sorghum juice supplemented with 0.5% ammonium sulphate was used as a substrate for ethanol production by Saccharomyces cerevisiae TISTR 5048. In batch fermentation, kinetic parameters for ethanol production depended on initial cell and sugar concentrations. The optimum initial cell and sugar concentrations in the batch fermentation were 1 × 108 cells ml−1 and 24 °Bx respectively. At these conditions, ethanol concentration produced (P), yield (Y ps) and productivity (Q p ) were 100 g l−1, 0.42 g g−1 and 1.67 g l−1 h−1 respectively. In fed-batch fermentation, the optimum substrate feeding strategy for ethanol production at the initial sugar concentration of 24 °Bx was one-time substrate feeding, where P, Y ps and Q p were 120 g l−1, 0.48 g g−1 and 1.11 g l−1 h−1 respectively. These findings suggest that fed-batch fermentation improves the efficiency of ethanol production in terms of ethanol concentration and product yield.
KeywordsBatch fermentation Fed-batch fermentation Ethanol production Saccharomyces cerevisiae Sweet sorghum
This research was financially supported by the National Research Council of Thailand (NRCT), Thailand. We would like to thank our students, Ms. Suparat Meekhun, Ms. Wannapa Glangsoom and Ms. Angkana Narongkasemsuk, for their technical assistance.
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