World Journal of Microbiology and Biotechnology

, Volume 23, Issue 9, pp 1233–1238 | Cite as

Modeling of lutein production by heterotrophic Chlorella in batch and fed-batch cultures

  • Zheng-Yun Wu
  • Chun-Lei Shi
  • Xian-Ming ShiEmail author
Full-length Paper


Chlorella is a promising alternative resource of lutein (xanthophyll) production as it can be cultivated heterotrophically in fermentors. In this paper, a kinetic model for lutein production by heterotrophic Chlorella pyrenoidosa was developed based on batch cultivations in 250-ml flasks and a 19-l fermentor. The model was validated by experimental data from two fed-batch cultivations performed in the same fermentor. The dynamic behavior of lutein production by C. pyrenoidosa with various concentrations of glucose and nitrogen was analyzed based on the kinetic model. Model-based analyses suggested that glucose concentrations between 5 and 24 g/l and nitrogen concentrations between 0.7 and 12 g/l during the cultivation were favorable for lutein production by heterotrophic C. pyrenoidosa. It also showed that fed-batch cultivations are more suitable for efficient production of lutein than batch ones. The results obtained in this study may contribute to commercial lutein production by heterotrophic Chlorella.


Lutein production Xanthophyll Chlorella pyrenoidosa Heterotrophic culture Kinetic model 



This research was supported by Ministry of Science and Technology of the People’s Republic of China (863 project No. 2006AA02Z226).


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Food Science and Technology, Bor Luh Food Safety CenterShanghai Jiao Tong UniversityShanghaiP. R. China

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