World Journal of Microbiology and Biotechnology

, Volume 23, Issue 8, pp 1131–1137 | Cite as

Immobilization of Aspergillus oryzae α-galactosidase in gelatin and its application in removal of flatulence-inducing sugars in soymilk

  • K. Naganagouda
  • S. J. Prashanth
  • S. K. Shankar
  • S. K. Dhananjay
  • V. H. MulimaniEmail author
Original Paper


Raffinose oligosaccharides (RO) are the major factors responsible for flatulence following ingestion of soybean-derived products. Removal of RO from seeds or soymilk would then have a positive impact on the acceptance of soy-based foods. In this study, α-galactosidase from Aspergillus oryzae was entrapped in gelatin using formaldehyde as the hardener. The immobilization yield was 64.3% under the optimum conditions of immobilization. The immobilized α-galactosidase showed a shift in optimum pH from 4.8 to 5.4 in acetate buffer. The optimum temperature also shifted from 50°C to 57°C compared with soluble enzyme. Immobilized α-galactosidase was used in batch, repeated batch and continuous mode to degrade RO present in soymilk. In the repeated batch, 45% reduction of RO was obtained in the fourth cycle. The performance of immobilized α-galactosidase was tested in a fluidized bed reactor at different flow rates and 86% reduction of RO in soymilk was obtained at 25 ml h−1 flow rate. The study revealed that immobilized α-galactosidase in continuous mode is efficient in reduction of RO present in soymilk.


α-Galactosidase Aspergillus oryzae Fluidized bed reactor Gelatin Raffinose oligosaccharides Soymilk 



One of the authors Naganagouda V. Kote thanks Gulbarga University, Gulbarga for providing the financial support in the form of University Ph.D. junior research fellowship.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • K. Naganagouda
    • 1
  • S. J. Prashanth
    • 2
  • S. K. Shankar
    • 1
  • S. K. Dhananjay
    • 1
  • V. H. Mulimani
    • 1
    Email author
  1. 1.Department of Biochemistry Gulbarga UniversityGulbargaIndia
  2. 2.Department of Biochemistry Indian Institute of ScienceBangaloreIndia

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