Rapid estimation of polysaccharide content in complex microbial culture media

  • Silas G. Villas-Bôas
  • Subathira Sivakumaran
  • Graeme Attwood
  • Geoffrey A. Lane
  • Adrian Cookson
Brief Communication

Abstract

A rapid gravimetric assay for estimation of polysaccharide content in complex culture media was developed and validated. The quantitative assay showed great selectivity for the determination of xylan and pectin, eliminating all interferences from other medium components. In addition, our data showed this protocol could also be applied to the estimation of other polysaccharides in liquid media such as cellulose Avicel®, chitin, starch and crude hemicellulose. Therefore, this method represents a useful tool for monitoring the in vitro microbial degradation of polysaccharides.

Keywords

Biopolymers Degradation Hydrolases Hydrolytic activity Quantification. 

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Silas G. Villas-Bôas
    • 1
  • Subathira Sivakumaran
    • 1
  • Graeme Attwood
    • 1
  • Geoffrey A. Lane
    • 1
  • Adrian Cookson
    • 1
  1. 1.AgResearch LimitedGrasslands Research CenterPalmerston NorthNew Zealand

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