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Influence of soil fertility on the rhizobial competitiveness for nodulation of Acacia senegal and Acacia nilotica provenances in nursery and field conditions

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Abstract

Within the framework of our study, we assessed the nodule occupancy of a mixture of various strains of rhizobia to inoculate several provenances of Acacia senegal and Acacia nilotica. The first part of the experiment was carried out under greenhouse conditions where the plants were cultivated in polyvinyl chloride tubes containing an unsterilized Sangalkam soil low in organic matter and nitrogen. The results showed that 4 and 8 months after sowing, rhizobial strains CIRADF 306 and CIRADF 300 were mainly present in nodules of A. nilotica and A. senegal, respectively. After transferring the seedlings to the more fertile soil in Bel Air field station, the molecular analysis of the nodules showed that strain CIRADF 306 was absent from the nodules of A. nilotica, whereas strain CIRADF 305 which occurred only at low nodule occupancy in the nursery, predominated in the field conditions. On the other hand, strain CIRADF 300 occurred in the majority of the nodules from the various provenances of A. senegal. These results demonstrated actual interaction between inoculated rhizobial strains, soil type and host plant genotype in terms of competitiveness, nodulation and symbiotic nitrogen fixation.

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Acknowledgments

This work was funded by the Forest Department of CIRAD. The authors are grateful to Jacques Biagui, Leon Biagui, Cheikh Ndiaye and Lamine Ba for their technical assistance in the nursery and in the field. We wish to thank Dr David Odee from the Kenyan Forestry Research Institute for reviewing the manuscript and editing the English.

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Correspondence to D. Lesueur.

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Sarr, A., Lesueur, D. Influence of soil fertility on the rhizobial competitiveness for nodulation of Acacia senegal and Acacia nilotica provenances in nursery and field conditions. World J Microbiol Biotechnol 23, 705–711 (2007). https://doi.org/10.1007/s11274-006-9288-0

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Keywords

  • Acacia provenances
  • Nodules occupancy
  • PCR-RFLP
  • Rhizobia
  • Soil fertility