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World Journal of Microbiology and Biotechnology

, Volume 23, Issue 1, pp 125–133 | Cite as

Identification and characterization of dominant lactic acid bacteria isolated from traditional fermented milk Dahi in Bangladesh

  • Md. Harun-ur-Rashid
  • Kaname Togo
  • Minoru Ueda
  • Taku MiyamotoEmail author
Original Paper

Abstract

We isolated a total of 266 strains of lactic acid bacteria (LAB) from 28 dahi samples that were collected from different areas in Bangladesh. The isolated strains were identified on basis of their morphological, physiological and biochemical characteristics, the lactic acid isomer produced, the ability to ferment sugars and 16S rDNA analysis. Among the isolates, the cocci (73%) were dominant over the rods (27%). The distribution of the isolates by genus was as follows: Streptococcus (50%), Lactobacillus (27%), Enterococcus (9%), Leuconostoc (5%), Lactococcus (5%) and Pediococcus (4%). In this study, S. bovis was the most predominant species as this species represents 47.0% of the total isolates in dahi. The other species we isolated were identified as Lb. fermentum, Lb. delbrueckii ssp. bulgaricus, Lb. delbrueckii ssp. lactis, Lb. sp., Ec. faecium, S. thermophilus, Leuc. mesenteroides ssp. mesenteroides, Leuc. mesenteroides ssp. dextranicum, Lc. lactis ssp. lactis, Lc. raffinolactis and P. pentosaceus.

Keywords

Lactic acid bacteria Traditional fermented milk Dahi 

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Notes

Acknowledgements

The authors wish to thank the Ministry of Education, Culture, Sports, Science and Technology, and Foods Research and Development Center, Kaneka Corporation, Japan for financial support.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Md. Harun-ur-Rashid
    • 1
    • 2
  • Kaname Togo
    • 1
  • Minoru Ueda
    • 3
  • Taku Miyamoto
    • 1
    Email author
  1. 1.Graduate School of Natural Science and TechnologyOkayama UniversityTsushima, OkayamaJapan
  2. 2.Department of Dairy ScienceBangladesh Agricultural UniversityMymensinghBangladesh
  3. 3.Foods R & D CenterKaneka CorporationTakasagoJapan

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