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Antimicrobial potentials of endophytic fungi residing in Quercus variabilis and brefeldin A obtained from Cladosporium sp.

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Abstract

Among 67 endophytic fungi isolated from Quercus variabilis, 53.7% of endophytic fungal fermentation broths displayed growth inhibition on at least one test microorganism, such as pathogenic fungi (Trichophyton rubrum, Candida albicans, Aspergillus niger, Epidermophyton floccosum, Microsporum canis) and bacteria (Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens). Moreover, 19.4% of strains showed a broader antimicrobial spectrum, such as Aspergillus sp., Penicillium sp., Alternaria sp., 20.9% of strains showed strong inhibition (+++) to pathogenic bacteria, while only 7.5% displayed that to test fungi. The most active antifungal strain I(R)9-2, Cladosporium sp. was selected and fermented. From the broth, a secondary metabolite, brefeldin A was obtained. This is the first report on the antimicrobial potentials of endophytic fungi residing in Q. variabilis and isolation of brefeldin A produced by Cladosporium sp.

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References

  1. Arnold AE, Maynard Z, Gilbert GS (2001) Fungal endophytes in dicotyledonous neotropical trees: patterns of abundance and diversity. Mycol Res 105:1502–1507

  2. Barchiesi F, Arzeni D, Fothergill AW, Di Francesco LF, Caselli F, Rinaldi MG, Scalise G (2000) In vitro activities of the new antifungal triazole SCH 56592 against common and emerging yeast pathogens. Antimicrob Agents Chemoth 44:226–229

  3. Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized Single disk method. Am J Clin Pathol 45:493–496

  4. Bills GF (1996) Isolation and analysis of endophytic fungal communities from woody plants. In: Redlin SC, Carris LM (eds), Endophytic fungi in grasses and woody plants. Systematics, ecology and evolution. APS Press, St. Paul, MN, USA, pp 31–65 ISBN: 0-89054-213-9

  5. Carroll GC (1988) Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 69:2–9

  6. Faeth SH, Hammon KE (1997) Fungal endophytes in oak trees: experimental analyses of interactions with leafminers. Ecol Soc Am 78:820–827

  7. Fisher PJ, Anson AE, Petrini O (1984) Antibiotic activity of some endophytic fungi from ericaceous plants. Bot Helv 94:249–253

  8. Harri E, LoeMer W, Singh HP, Stahlin H, Tamm C (1963) Die Constitution von Brefeldin A. Helv Chem Acta 46:1235–1243

  9. Hawksworth DL (1988) The variety of fungal-algal symbioses, their evolutionary significance, and the nature of lichens. Bot J Linn Soc 96:3–20

  10. Liu CH, Meng JC, Zou WX, Huang LL, Tang HQ, Tan RX (2002) Antifungal metabolite with a new carbon skeleton from Keisslerriella sp. YS4108, a marine filamentous fungus. Planta Med 68:363–365

  11. Liu CH, Zou WX, Lu H, Tan RX (2001) Antifungal activity of Artemisia annua endophyte cultures against phytopathogenic fungi. J Biotechnol 88:277–282

  12. Liu JY, Song YC, Zhang Z, Wang L, Guo ZJ, Tan RX (2004) Aspergillus fumigatus, CY018, an endophytic fungus in Cynodon dactylon as a versatile producer of new and bioactive metabolites. J Biotechnol 114:279–287

  13. Maurizio V, Antonio E, Anna A, Maria CZ, Federico G, Andrea M (1998) Brefeldin A and α, β-dehydrocurvularin, two phytotoxins from Alternaria zinniae, a biocontrol agent of Xanthium occidentale. Plant Sci 138:67–79

  14. McGee PA, Hincksman MA, White CS (1991) Inhibition of growth of fungi isolated from plants by Acremonium strictum. Aust J Agric Res 42:1187–1194

  15. Schulz B, Sucker J, Aust HJ, Krohn K, Ludewig K, Jones PG, Doring D (1995) Biologically active secondary metabolites of endophytic Pezicula species. Mycol Res 99:1007–1015

  16. Strobel GA, Long DM (1998) Endophytic microbes embody pharmaceutical potential. ASM News 64:263–268

  17. Tan RX, Zou WX (2001) Endophytes: a Rich source of functional metabolites, The Royal Society of Chemistry. Nat Prod Rep 18:448–459

  18. Wang JF, Huang YJ, Fang MJ, Zhang YJ, Zheng ZH, Zhao YF, Su WJ (2002) Brefeldin A, a cytotoxin produced by Paecilomyces sp and Aspergillus clavatus isolated from Taxus mairei and Torreya grandis. FEMS Immunol Med Microbiol 34:51–57

  19. Weber, Roland WS, Stenger E, Meffert A, Hahn M (2004) Brefeldin A production by Phoma medicaginis in dead pre-colonized plant tissue: a strategy for habitat conquest? Mycol Res 108:662–671

  20. Zhang WH, Duan BL, Zhou JY, Liu XJ (2004) Water relations and activity of cell defense enzymes to water stress in seedling leaves of different provenances of Quercus variabilis. Zhiwu Shengtai Xuebao 28:483–490

  21. Zhou LH, Sun QS, Qiao LC, Liu J (2000) Preliminary Studies on the Chemical Constituents in the Active Section of the Leaves of Quercus variabilis Blume. J Shenyang Pharm Univ 17:179–181

  22. Zhou LH, Sun QS, Wang Y (2003) Two new cycloartane triterpenes from the leaves of Quercus variabilis Blume. Chinese Chem Lett 14:1265–1267

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Acknowledgements

The work was co-supported by the Ministry of Education (Key Project No: 104195), by NSFC (30300007) and by JSNSF (BK2003410).

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Correspondence to Y. C. Song.

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Wang, F.W., Jiao, R.H., Cheng, A.B. et al. Antimicrobial potentials of endophytic fungi residing in Quercus variabilis and brefeldin A obtained from Cladosporium sp.. World J Microbiol Biotechnol 23, 79–83 (2007). https://doi.org/10.1007/s11274-006-9195-4

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Keywords

  • Endophytic fungi
  • Antimicrobial potentials
  • Quercus variabilis
  • Cladosporium sp. brefeldin A