This study was designed to examine the reaction of microbial communities to the associated impact of inorganic fertilizers and pesticides during laboratorial simulation for 90 days. Soils were sampled from fields in three different stations of Chinese Ecosystem Research Network (CERN). The metabolic profiles were characterized by using BIOLOG GN2 microplates. The Shannon Index (H) showed that the metabolic diversity of the three types of soil exhibited similar profiles. PCA and CLUSTER further explored the variance in community structures with the same H. There were obvious changes in the structures of microbial communities during the 90-day duration of the work and the three different types of soil showed␣similar profiles for most of the period of the experiment.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Avidano L, Gamalero E, Cossa GP, Carraro E (2005) Characterization of soil health in an Italian polluted site by using microorganisms as bioindicators. Appl Soil Ecol 30:21–33
Bossio DA, Scow KM (1995) Impact of carbon and flooding on the metabolic diversity of microbial communities in soils. Appl Environ Microbiol 61:4043–4050
Cheng CC (2004) Statistical approaches on discriminating spatial variation of species diversity. Bot Bull Acad Sinica 45:339–346
Engelen B, Meinken K, Von Wintzingerode F, Heuer H, Malkomes HP, Backhaus H (1998) Monitoring impact of a pesticide treatment on bacterial soil communities by metabolic and genetic fingerprinting in addition to conventional testing procedures. Appl Environ Microbiol 64:2814–2821
Garland JL (1996) Analytical approaches to the characterization of samples of microbial communities using patterns of␣potential C source utilization. Soil Biol Biochem 28:213–221
Garland JL, Mills AL (1991) Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl Environ Microbiol 57:2351–2359
Garland JL, Mills AL, Young JS (2001) Relative effectiveness of kinetic analysis vs single point readings for classifying environmental samples based on community-level physiological profiles (CLPP). Soil Biol Biochem 33:1059–1066
Grayston SJ, Wang SQ, Campbell CD, Edwards AC (1998) Selective influence of plant species on microbial diversity in the rhizosphere. Soil Biol Biochem 30:369–378
Haack SK, Garchow HM, Klug J, Forney LJ (1995) Analysis of factors affecting the accuracy, reproducibility, and interpretation of microbial community carbon source utilization patterns. Appl Environ Microbiol 61:1458–1468
Hald PM (1947) The flame photometer for the measurement of sodium and potassium in biological materials. J Biol Chem 167:499–510
Lindstrom JE, Barry RP, Braddock JF (1998) Microbial community analysis: a kinetic approach to constructing potential C source utilization patterns. Soil Biol Biochem 30:231–239
Lovell RD, Jarvis SC, Bardgett RD (1995) Soil microbial biomass and activity in long-term grassland: effects of management change. Soil Biol Biochem 27:969–975
Nannipieri P, Ascher J, Ceccherini MT, Landi L, Pietramellara G, Renella G (2003) Microbial diversity and soil functions. Eur J Soil Sci 54:655–670
Oȁ9Donnell AG, Seasman M, Macrae A, Waite I, Davies JT (2001) Plants and fertilisers as drivers of change in microbial community structure and function in soils. Plant Soil 232:135–145
Preston-Mafham J, Boddy L, Randerson PF (2002) Analysis of microbial community functional diversity using sole-carbon-source utilization profiles—a critique. FEMS Microbiol Ecol 42:1–14
Rogers BF, Tate RL III (2001) Temporal analysis of the soil microbial community along a toposequence in Pineland soils. Soil Biol Biochem 33:1389–1401
Rölleke S, Gurter C, Drevello U, Drewello R, Lubitz W, Weissmann R (1999) Analysis of bacterial communities on historical glass by denaturing gradient gel electrophoresis of␣PCR-amplified gene fragments coding for 16S rRNA. J␣Microbiol Methods 36:107–114
Sarathchandra SU, Ghani A, Yeates GW, Butch G, Cox NR (2001) Effect of nitrogen and phosphate fertilizers on microbial and nematode diversity in pasture soils. Soil Biol Biochem 33:953–964
Thirup L, Johnsen K, Torsvik V, Spliid NH, Jacobsen CS (2001) Effects of fenpropimorph on bacteria and fungi during decomposition of barley roots. Soil Biol Biochem 33:1517–1524
Wardle DA (1992) A comparative assessment of factors which influence microbial biomass carbon and nitrogen levels in soil. Biol Rev Camb Philos Soc 67:321–358
Wünsche L, Brüggemann L, Babel W (1995) Determination of substrate utilization patterns of soil microbial communities: an approach to assess population changes after hydrocarbon pollution. FEMS Microbiol Ecol 17:295–306
Yang YH, Yao J, Hu S, Qi Y. (2000) Effects of agricultural chemicals on DNA sequence diversity of soil microbial community: a study with RAPD marker. Microb Ecol 39:72–79
Zak JC, Willing MR, Moorhead DL, Wildman HG (1994) Functional diversity of microbial communities: a quantitative approach. Soil Biol Biochem 26:1101–1108
Zheng H, Ouyang ZK, Wang XK, Fang ZG, Zhao TQ, Miao H (2005) Effects of regenerating forest cover on soil microbial communities: a case study in hilly red soil region, Southern China. For Ecol Manage 217:244–254
This work was supported by the National Science Foundation of China (NSFC, Project No. 30470289 and 20477051).
About this article
Cite this article
Chen, J., Zhuang, X., Xie, H. et al. Associated impact of inorganic fertilizers and pesticides on microbial communities in soils. World J Microbiol Biotechnol 23, 23–29 (2007). https://doi.org/10.1007/s11274-006-9189-2
- Inorganic fertilizers
- Metabolic diversity