Two Phosphate- and Potassium-solubilizing Bacteria Isolated from Tianmu Mountain, Zhejiang, China
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Two phosphate- and potassium-solubilizing strains (KNP413 and KNP414) were isolated from the soil of Tianmu Mountain, Zhejiang Province (China) and they were phenotypically and phylogenetically characterized. Both isolates effectively dissolved mineral phosphate and potassium, while strain KNP414 showed higher dissolution capacity even than Bacillus mucilaginosus AS1.153, the inoculant of potassium fertilizer widely used in China. When grown on Aleksandrov medium, both strains were rod-shaped spore-formers with a large capsule, and they formed slimy and translucent colonies. The DNA G+C contents were 57.7 mol% for strain KNP413 and 56.1 mol% for strain KNP414. Strain KNP413 shared a 16S rRNA gene sequence similarity of more than 99.1% with strain KNP414 and Bacillus mucilaginosus strains HSCC 1605 and YNUC0001, and a 94.6% similarity with Bacillus mucilaginosus VKM B-1480D, the type strain of Bacillus mucilaginosus. Strains KNP413 and KNP414 together with other Bacillus mucilaginosus were clustered with Paenibacillus strains in a group. The use of a specific PCR primer PAEN515F designed for differentiating the genus Paenibacillus from other members of the Bacillaceae showed that strains KNP413 and KNP414 had the same amplified 16S rRNA gene fragment (0.9-kb) as members of the genus Paenibacillus. In conclusion, phosphate- and potassium-solubilizing strains KNP413 and KNP414 should be integrated into the same species different from strain VKM B-1480D and they might be transferred to the genus of Paenibacillus, i.e. Paenibacillus mucilaginosus.
Key wordsBacillus mucilaginosus identification Paenibacillus phosphate and potassium solubilization silicate bacteria
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The studies of morphology and DNA G+C were performed at the Analysis Center of Zhejiang University. This research was financial supported by the National Foundation of Science, P.R. China (Project No. 021102022).
- Aleksandrov V.G., Blagodyr R.N., Ilev I.P., 1967 Liberation of phosphoric acid from apatite by silicate bacteria Mikrobiolohichnyi Zhurnal (Kiev) 29: 111–114Google Scholar
- Avankyan Z.A., Pivovarova T.A., Karavaiko G.I., 1986 Properties of a new Species, Bacillus mucilanginosus Mikrobiologiya 55: 477–482Google Scholar
- Claus D., Berkeley R.C.W., 1986 Genus Bacillus Cohn 1872 In: Sneath P.H.A., Mair N.S., Sharpe M.E., Holt J.G. (ed) Bergey’s Manual of Systematic Bacteriology Williams & Wilkins Baltimore pp. 1105–1139 ISBN 0–683–07893–3Google Scholar
- Fiske C.H., Subbarow Y., 1925 A colorimetric determination of phosphate Journal of Biological Chemistry 66: 375–400Google Scholar
- Gaur A.C., Ostwal K.P., 1972 Influence of phosphate dissolving Bacilli on yield and phosphate uptake of wheat crop Indian Journal of Experimental Biology 10: 393–394Google Scholar
- Goldstein A.H., 1986 Bacterial solubilization of mineral phosphates: historical perspective and future prospects American Journal of Alternative Agriculture 1: 51–57Google Scholar
- Goldstein A.H., 1994 Involvement of the quinoprotein glucose dehydrogenase in the solubilization of exogenous mineral phosphates by gram-negative bacteria In: Torriani-Gorni A., Yagil E., Silver S., (ed) Phosphate in Microorganisms: Cellular and Molecular Biology ASM Press Washington pp. 197–203 ISBN 1555810802Google Scholar
- Manib M., Zahra M.K., Abdel A.L., Heggo A., 1986 Role of silicate bacteria in releasing K and Si from biotite and orthoclase In: Szegi J., (Ed.) Soil biology and Conservation of the Biosphere Akademiai Kiado Budapest pp. 733–743 ISBN 9630537001Google Scholar
- Nianikova G.G., Kuprina E.E., Pestova O.V., Vodolazhskaia S.V., 2002 Immobilizing of Bacillus mucilaginosus, a producer of exopolysaccharides, on chitin Prikladnaia Biokhimiia i Mikrobiologiya 38: 300–304Google Scholar
- Shelobolina E.S., Avakyan Z.A., Boulygina E.E., Turova T.P., Lysenko A.M., Osipov G.A., Karavaiko G.I., 1997 Description of a new species of mucilaginosus bacteria, Bacillus edaphicus sp. Nov. and confirmation of the taxonomic status of Bacillus mucilaginosus Mikrobiologiya 66: 813–822Google Scholar
- Sheng X.F., Huang W.Y., 2002. Mechanism of potassium release from feldspar affected by the strain nbt of silicate bacterium Acta Pedologica Sinica 39: 863–871Google Scholar
- Sheng X.F., Huang W.Y., Yin Y.X., 2003 Effects of application of silicate bacteria fertilizer and its potassium release Journal of Nanjing Agricultural University 23: 43–46Google Scholar
- Shida O., Takagi H., Kadowaki K., Nakamura L.K., Komagata K., 1997 Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the Genus Paenibacillus and Emended Description of the Genus Paenibacillus International Journal of Systematic Bacteriology 47: 289–298PubMedGoogle Scholar
- Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., Stackebrandt E., Starr M.P., Trüper H.G., 1987 Report of the Ad Hoc committee on reconciliation of approaches to bacterial systematics International Journal of Systematic Bacteriology 37: 463–464CrossRefGoogle Scholar