World Journal of Microbiology and Biotechnology

, Volume 22, Issue 9, pp 901–907 | Cite as

Treatment of Paenibacillus illinoisensis suppresses the activities of antioxidative enzymes in pepper roots caused by Phytophthora capsici infection

  • W. J. Jung
  • Y. L. Jin
  • R. D. Park
  • K. Y. Kim
  • K. T. Lim
  • T. H. Kim
Article

Summary

The activity of antioxidative enzymes after inoculation of pepper (Capsicum annuum L. Chungok) with a pathogen, Phytophthora capsici (P), the causal agent of Phytophtora blight and dual inoculation of pathogen and an antagonist, Paenibacillus illinoisensis KJA-424 (P+A), were measured and compared with that of non-inoculated (C) roots. Root mortality was significantly reduced by about 84% in P+A treatment compared with P treatment alone. When compared to the non-inoculated (C) roots, malondialdehyde (MDA) concentration gradually decreased by 52.4% in 7 days only in P-treated roots and hydrogen peroxide (H2O2) was not significantly affected by the treatment for 5 days but significantly decreased in the P+A-treated roots at day 7. P-treatment continuously induced peroxidase (POD) and superoxide dismutase (SOD), resulting in significant increases of 36.7% and 27.7% at day 7, respectively, compared to the control. In P+A-treated roots, the activities of POD and SOD also increased for 5 days but returned to the control level at day 7. Catalase activity fluctuated but again increased over the 7-day period following P+A inoculation. These results indicate that an antagonist P. illinoisensis KJA-424 alleviated root mortality and suppressed the elevated activities of POD and SOD in the root of pepper plant root caused by P.␣capsici infection.

Keywords

Catalase Paenibacillus illinoisensis pepper peroxidase Phytophthora capsici superoxide dismutase 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • W. J. Jung
    • 1
  • Y. L. Jin
    • 1
  • R. D. Park
    • 1
  • K. Y. Kim
    • 1
  • K. T. Lim
    • 2
  • T. H. Kim
    • 3
  1. 1.Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, and APSRC, College of Agriculture & Life ScienceChonnam National UniversityGwangjuKorea
  2. 2.# 521 Molecular Biochemistry Laboratory & Biodefensive Substances GroupChonnam National UniversityGwangjuKorea
  3. 3.Department of Animal Science, and Institute of Agricultural Science & Technology, College of Agriculture & Life ScienceChonnam National UniversityGwangjuKorea

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