Studies on the kinetic parameters for alcoholic fermentation by flocculent Saccharomyces cerevisiae strains and non-hydrogen sulfide-producing strains
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Summary
The growing demand for high quality products and the immense export potential that cachaça represents, demonstrated especially during the past few years, have clearly indicated the necessity of establishing well-defined standards of quality, as well as effective means of controlling the process of production of this beverage. The objective of this study was the selection of S. cerevisiae yeast strains and the investigation of their influence on the kinetic parameters of fermentation. Ninety strains of S. cerevisiae isolated from distilleries of the state of Minas Gerais were evaluated with respect to the following parameters: flocculation capacity, production of H2S and kinetic parameters of fermentation. The UFMGA 905 strain was used as a reference because it presented desirable characteristics for the production of cachaça. Five strains presented high specific sedimentation velocities (SSV), indicating a high flocculation capacity, and two did not produce H2S. The strains presented significant statistical differences for fermentation parameters: yield of ethanol; efficiency of substrate conversion to ethanol; ratio of substrate conversion to ethanol (Y p/s), to cells (Y x/s), to organic acids (Y ac/s), and to glycerol (Y g/s); and productivity. In general, the strains presented a good fermentative potential, with ethanol yields varying from 74.7 to 82.1% and an efficiency of 76.1–84.4%. All strains presented high productivities (4.6–6.6 g l−1 h−1), indicating that this parameter can be used in the selection of strains for the production of cachaça.
Keywords
Cachaça flocculent kinetic parameters Saccharomyces cerevisiae yeastPreview
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Notes
Acknowledgements
This work was funded by Fundação do Amparo a Pesquisa de Minas Gerais (FAPEMIG – process no. CBB-334/02) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
References
- Alves, D.M.G. 1994 Fatores que afetam a formação de ácidos orgânicos bem como outros parâmetros. MS thesis, Esalq, Piracicaba:, BrazilGoogle Scholar
- Andrietta S.R., Miligliari P.C., Andrietta M.G.S., 1999 Classificação das cepas de levedura de processos industriais de fermentação alcoólica utilizando capacidade fermentativa Sociedade. Tecnologia de Álcool e Bebidas, Piracicaba, 17: 54–59Google Scholar
- Bayly J.C., Douglas L.M., Pretorius I.S., Bauer F.F., Dranginis A.M., 2005 Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae FEMS Yeast Research, 5: 1151–1156CrossRefPubMedGoogle Scholar
- Berry, D.R. 1995 Alcoholic beverage fermentations. In Fermented Beverage Production, eds. Lea A.G.H. & Piggott J.R. 1st. edn. pp.␣32–54 London. Blackie Academic & Professional, ISBN 0751400270Google Scholar
- Bony M., Thines-Sempoux D., Barre P., Blondin B., 1997 Local-isation and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p Journal of Bacteriology 179: 4929–4936PubMedGoogle Scholar
- Gutierrez L.E. 1991 Produção de álcoois superiores por linhagens de Saccharomyces durante a fermentação alcoólica Science Agricultural 50: 464–472Google Scholar
- Macgowan M.W., Artiss J.D., Strandbergh D.R., Zak B., 1983 A peroxidase-coupled method for the colorimetric determination of serum triglycerides Clinical Chemistry 29: 538–542PubMedGoogle Scholar
- Mendonça, A.T. 1999 Identificação e estudo das características fisiológicas de S. cerevisiae presentes em fermentação espontânea de cana-de-açúcar. MS thesis, UFL, Lavras, BrazilGoogle Scholar
- Miller G.L., 1959 Use of dinitrosalicylic acid reagent for determination of reducing sugar Analytical Chemistry 31: 426–428CrossRefGoogle Scholar
- Morais P.B., Rosa C.A., Linardi V.R., Pataro C., Maia A.B.R.A., 1997 Characterization and succession of yeast populations associated with spontaneous fermentations during the production of brazilian sugar-cane cachaça World Journal of Microbiology and Biotechnology 13: 241–243CrossRefGoogle Scholar
- Oliveira E.S., Rosa C.A., Morgano M.A., Serra G.E., 2004 Fermentation characteristics as criteria for selection of cachaça yeast World Journal of Microbiology and Biotechnology 20: 19–24CrossRefGoogle Scholar
- Oliveira E.S., Cardello H.N.A.B., Jerônimo E.M., Souza E.L.R., Serra G.E., 2005 The influence of different yeast fermentation, composition and sensory quality of cachaça World Journal of Microbiology and Biotechnology 21: 707–715CrossRefGoogle Scholar
- Parazzi, C. 1995 Fermentação alcoólica com leveduras floculantes. MS thesis, Unesp, Rio claro, BrasilGoogle Scholar
- Pataro C., Santos A., Corrêa S.R., Morais P.B., Linardi V.R., Rosa C.A., 1998 Physiological characterization of yeasts isolated from artisanal fermentation in an cachaça distillery Revista de Microbiologia 29: 69–73Google Scholar
- Pataro C., Guerra J.B., Petrillo-Peixoto M.L., Mendonça-Hagler L.C., Linardi V.R., Rosa C.A., 2000 Yeast communities and genetic polymorphism of Saccharomyces cerevisiae strains associated with artisanal fermentation in Brazil Journal of Applied Microbiology 88: 1–9CrossRefGoogle Scholar
- Pimentel-Gomes, F. (Ed.) 2000 Curso de estatística experimental. Piracicaba: 14th edn., 477pGoogle Scholar
- Radler, F.,Schutz, H. 1982 Glycerol Production ofvarious strains of saccharomyces. AmericanJournal of Enology and Viticulture 33, 36–40Google Scholar
- Rankine B.C., Bridson D.A., 1971 Glycerol in Australian wines and factors influencing its formation American Journal of Enology and Viticulture, 22: 6–12Google Scholar
- Ribeiro, C.A.F. 1997 Potencialidades de diferentes linhagens de levedura da espécie Saccharomyces cerevisiae na tecnologia de cachaça de cana. MS thesis, Esalq, Piracicaba, BrasilGoogle Scholar
- Stewart G.G., Garrison I.F., Goring T.E., Meleg M., Piparts P., Russel I., 1976 Biochemical and genetic studies on yeast flocculation Kemia-Kemi 3: 465–479Google Scholar
- Stratford M., Coleman H.P., Keenan M.H.J., 1988 Yeast flocculation: a dynamic equilibrium Yeast 4: 199–208 CrossRefPubMedGoogle Scholar
- Verstrepen K.J., Derdelinckx G., Verachtert H., Delvaux F.R., 2003 Yeast flocculation: what brewers should know Applied Microbiology and Biotechnology 61: 197–205PubMedGoogle Scholar
- Viegas, M.C. 1996 Desenvolvimento de um reator de bancada de leito fluidizado para produção de etanol utilizando linhagens de leveduras floculantes. MS thesis, Unesp, Campinas, BrazilGoogle Scholar
- Zambonelli C., 1964a Ricerche biometriche sulla produzione di idrogeno solforato da solfati e solfiti in Saccharomyces cerevisiae var. ellipsoideus Annali di microbiologia ed enzimologia 14: 129–41Google Scholar
- Zambonelli C., 1964b Ricerche genetiche sulla produzione di idrogeno solforato in Saccharomyces cerevisiae var ellipsoideus Annali di microbiologia ed enzimologia 14: 143–53Google Scholar
- Zimmermann H.W., 1963 Studies on the dicromate method of alcohol determination American Journal of Enology and Viticulture 14: 205–213Google Scholar