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Heterologous Expression and Purification of Recombinant Allophycocyanin in Marine Streptomyces sp. Isolate M097

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Summary

Allophycocyanin is one of the most important marine active peptides. Previous studies suggested that recombinant allophycocyanin (rAPC) could remarkably inhibit the S-180 carcinoma in mice, indicating its potential pharmaceutical uses. Based on intergeneric conjugal transfer, heterologous expression of rAPC was first achieved in marine Streptomyces sp. isolate M097 through inserting the apc gene into the thiostrepton-induced vector pIJ8600. The transformation frequency for this system was approximately 10−4 exconjugants/recipient. In the transformed Streptomyces sp. isolate M097, the yield of purified rAPC could amount to about 38 mg/l using a simple purification protocol, and HPLC analysis showed that the purity of the protein reached about 91.5%. In vitro activity tests also revealed that the purified rAPC had effective scavenging abilities on superoxide and hydroxyl radicals. This would widen the usefulness of the marine Streptomyces as a host to express the rAPC and to offer industrial strain for the production of rAPC.

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Acknowledgements

We thank Prof Keith F. Chater, John Innes Institute, for the gift of the strain ET12567 (PUZ8002) and plasmid pIJ8600. This work was supported by National High Technology Research and Development Program of China (863 Program, 2003AA624020) and Key Innovative Project (KSCX2-SW-215) of the Chinese Academy of Sciences.

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Correspondence to Song Qin.

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Hou, Y., Qin, S., Li, Y. et al. Heterologous Expression and Purification of Recombinant Allophycocyanin in Marine Streptomyces sp. Isolate M097. World J Microbiol Biotechnol 22, 525–529 (2006). https://doi.org/10.1007/s11274-005-9067-3

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Keywords

  • Allophycocyanin
  • conjugal transfer
  • heterologous expression
  • marine
  • purification
  • Streptomyces