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Endophytic fungi of teak leaves Tectona grandis L. and rain tree leaves Samanea saman Merr.

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Summary

In order to study the foliar endophytes from teak (Tectona grandis L.) and rain tree (Samanea saman Merr.) growing in the campus of Chulalongkorn University, healthy leaves were collected at two-monthly intervals during January to December. The number of genera and species, together with their colonization frequency (CF%) in mature teak and rain tree leaves were greater than those in the young leaves. More endophytic isolates in the leaves of both trees were recovered during the rainy season. The fungal genera found in both young and mature teak leaves were Alternaria, Colletotrichum, Nigrospora, Phomopsis and mycelia sterilia. Phomopsis was the dominant genus in both young (newly emerged) and mature leaves. Fusarium, Penicillium, Schizophyllum commune and members of the Xylariaceae were found only in mature leaves. For the rain tree leaves, species of Phomopsis and mycelia sterilia were found in both young newly emerged and mature leaves. Colletotrichum and Penicillium were found only in mature leaves, whereas Nigrospora was found only in young newly emerged leaves. In this study, Phomopsis was the dominant genus in the leaves of both tree species. A total of 37 isolates of endophytic fungi isolated from teak and rain tree leaves were tested for the production of antimicrobial activities. Out of these, 18 isolates could produce inhibitory substances effective against Bacillus subtilis, Staphylococcus aureus and Escherichia coli and 3 isolates inhibited growth of Candida albicans in vitro.

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References

  1. Azevedo J.L., Maccheroni W., Jr., Pereira J.O., Araujo W.L. 2000. Endophytic microorganisms: a review on insect control and recent advances on tropical plants Electronic Journal of Biotechnology 3: 40–65

  2. Barnett, H.L., Hunter B.B. 1998 Illustrated Genera of Imperfect Fungi. New York: MacMillan Publ. Co ISBN: 0-89054-192-2

  3. Blodgett J.T., Swart W.J., Louw S.M., Weeks W.J. 2000. Species composition of endophytic fungi in Amaranthus hybridus leaves, petioles, stems and rootsMycologia 92: 853–859

  4. Bussaban B., Lumyoung S., Lumyoung P., McKenzie E.H.C., Hyde K.D. 2001. Endophytic fungi from Amomum siamenseCanadian Journal of Botany 74: 103–114

  5. Bills G.F., 1996. Isolation and analysis of endophytic fungal communities from woody plants. In: Redlin S.C., Carris L.M., (eds). Endophytic Fungi in Grasses and Woody Plants Systematics, Ecology, and Evolution. APS Press, St. Paul 31–65 ISBN 0-89054-213-9

  6. Fisher P.J., Petrini O., Petrini L.E., Sutton B.C. 1994. Fungal endophytes from the leaves and twigs of Quercus ilex L. from england, Majorca and SwitzerlandNew Phytologist 127: 133–137

  7. Fisher P.J., Petrini O., Sutton B.C. 1993. A comparative study of fungal endophytes in leaves, xylem and bark of Eucalyptus in Australia and England Sydowia 45: 338–345

  8. Frohlich J., Hyde K.D., Petrini O. 2000. Endophytic fungi associated with palms. Mycological Research 104: 1202–1212

  9. Hata K., Atari R., Sone K. 2002. Isolation of endophytic fungi from leaves of Pasania edulis and their within-leaf distributionsMycoscience 43: 369–373

  10. Huang Y., Wang J., Li G., Zheng Z., Su W. 2001. Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxus mairei, Cephalataxus fortunei and Torreya grandisFEMS Immunology and Medical Microbiology 31: 163–167

  11. Kumaresan V., Suryanarayanan T.S. 2001. Occurrence and distribution of endophytic fungi in a mangrove communityMycological Research 11:1388–391

  12. Lumyong S., Thongantha S., Lumyong P., Tomita F. 2000. Endophytic fungi from 13 bamboo species in ThailandBiotechnology for Sustainable Utilization of Biological Resources in the Tropics 14: 96–101

  13. Mekkamol, S. 1998 Endophytic fungi in Tectona grandis L. (Teak). PhD thesis, John Moores University, Liverpool, England

  14. Mekkamol S., Jonglaekha N., HywelJones N.L., Whalley A.J.S. 1996. Endophytic fungi of teak in Thailand: a preliminary accountBIOTEC NEWS 3: 4–5

  15. Pereira J.O., Azevedo J.L., Petrini O. 1993. Endophytic fungi of Stylosanthes: a first reportMycologia 85: 362–364

  16. Petrini O., Carroll G. 1981. Endophytic fungi in foliage of some Cupressaceae in OregonCanadian Journal of Botany 59: 629–636

  17. Photita W., Lumyong S., Lumyong P., Hyde K.D. 2001. Endophytic fungi of wild banana (Musa acuminata) at Doi Suthep Pui National Park, ThailandMycological Research 105: 1508–1513

  18. Ragazzi A., Moricca S., Capretti P., Dellavalle I., Torco E. 2003. Differences in composition of endophytic mycobiota in twigs and leaves of healthy and declining Quercus species in ItalyForest Pathology 33: 31–38

  19. Rodrigues K.F., 1994. The foliar fungal endophytes of the Amazonian palm Euterpe oleraceaMycologia 86: 376–385

  20. Rodrigues K.F., Samuels G.J. 1990. Preliminary study of endophytic fungi in a tropical palmMycological Research 94: 827–830

  21. Skerman P.J., Cameron D.G., Riveros F. 1988. Tropical Forage Legumes. 2nd edn. Food and Agriculture Organization of the United Nations, Italy. ISBN 9251021902

  22. Stierle A., Strobel G., Stierle D., 1993. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yewScience 260: 214—216

  23. Sutton B.C. 1980. The Coelomycetes. Commonwealth Mycological Institute Kew. ISBN 0-89054-213-9

  24. Stinson M., Ezra D., Hess W.M., Sears J., Strobel G., 2003. An endophytic Gliocladium sp of Eucryphia cordifolia producing selective volatile antimicrobial compoundsPlant Science 165: 913--922

  25. Strobel G.A. 2002. Rainforest endophytes and bioactive productsCritical Reviews in Biotechnology 22: 325–333

  26. Suryanarayanan T.S., Murali T.S., Venkatesan G. 2002. Occurrence and distribution of fungal endophytes in tropical forests across a rainfall gradient Canadian Journal of Botany 80: 818–826

  27. Tanaka N., Hamazaki T., Vacharangkura T. 1998. Distribution, growth and site requirments of TeakJapan Agricultural Research Quarterly 32: 65–77

  28. Thienhirun, S. 1997 A preliminary account of the Xylariaceae of Thailand. PhD thesis, John Moores University, Liverpool, England

  29. Wilson, D. & Carroll, G.C. 1994 Infection studies of Discula quercina, an endophyte of Quercus garryana. 86, 635–647

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Acknowledgements

We wish to thank Graduate School Chulalongkorn University for partial financial support.

Author information

Correspondence to Sukanyanee Chareprasert or Prakitsin Sihanonth.

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Chareprasert, S., Piapukiew, J., Thienhirun, S. et al. Endophytic fungi of teak leaves Tectona grandis L. and rain tree leaves Samanea saman Merr. . World J Microbiol Biotechnol 22, 481–486 (2006). https://doi.org/10.1007/s11274-005-9060-x

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Keywords:

  • Antimicrobial
  • endophytes
  • Samanea
  • Tectona