Optimization of conditions for cell cultivation of Porphyra haitanensis conchocelis in a bubble-column bioreactor
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Process conditions for cell cultures derived from conchocelis of female red macroalga Porphyra haitanensis were optimized in an illuminated 0.3-l bubble-column photobioreactor, using CO2 in air as the sole carbon source during a 20-day cultivation period. It reached the highest growth rate when the initial cell density was 700 mg l−1 (dry weight), the optional aeration rate was 1.2 v/v/min, inorganic nitrate concentration was 15 mM and inorganic phosphate concentration was 0.6 mM. This is the first reported bioreactor cultivation study of cell cultures derived from conchocelis of Porphyra haitanensis.
KeywordsBiomass bubble-column conchocelis optimization photobioreactor Porphyra haitanensis
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This work was supported by the grant from the Key Innovative Project of the Chinese Academy of Sciences (No. KZCX3-SW-215). The authors also thank Dr. JiangPeng for helpful discussions and Dr. YangRui for providing Porphyra haitanensis female conchocelis cells in this research.
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