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Effect of nitrogen source on lignocellulolytic enzyme production by white-rot basidiomycetes under solid-state cultivation

  • Eva Kachlishvili
  • Michel J. PenninckxEmail author
  • Nino Tsiklauri
  • Vladimir Elisashvili
Article

Summary

The effect of additional nitrogen sources on lignocellulolytic enzyme production by four species of white-rot fungi (Funalia trogii IBB 146, Lentinus edodes IBB 363, Pleurotus dryinus IBB 903, and P. tuberregium IBB 624) in solid-state fermentation (SSF) of wheat straw and beech tree leaves was strain- and substrate-dependent. In general, the yields of hydrolytic enzymes and laccase increased by supplementation of medium with an additional nitrogen source. This stimulating effect of additional nitrogen on enzyme accumulation was due to higher biomass production. Only xylanase specific activity of P. dryinus IBB 903 and laccase specific activity of L. edodes IBB 363 increased significantly (by 66% and 73%, respectively) in SSF of wheat straw by addition of nitrogen source to the control medium. Additional nitrogen (20 mM) repressed manganese peroxidase (MnP) production by all fungi tested. The study of the nitrogen concentration effect revealed that 10 mM peptone concentration was optimal for cellulase and xylanase accumulation by P. dryinus IBB 903. While variation of the peptone concentration did not cause the change in MnP yield, elevated concentrations of this nutrient (20–40 mM) led to a 2–3-fold increase of P. dryinus IBB 903 laccase activity. About 10–20 mM concentration of NH4NO3 was optimal for cellulase and xylanase production by F. trogii IBB 146. However, neither the laccase nor the MnP yield was significantly changed by the additional nitrogen source.

Key words

Cellulase Funalia trogii laccase Lentinus edodes manganese peroxidase nitrogen source Pleurotus dryinus P. tuberregium solid-state fermentation xylanase 

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Notes

Acknowledgments

Financial support from Government of the Brussels-Capital Region in the frame of programme ‘Research in Brussels’ is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Eva Kachlishvili
    • 1
  • Michel J. Penninckx
    • 2
    Email author
  • Nino Tsiklauri
    • 1
  • Vladimir Elisashvili
    • 1
  1. 1.Durmishidze Institute of Biochemistry and BiotechnologyAcademy of Sciences of GeorgiaTbilisiGeorgia
  2. 2.Faculte des Sciences, Laboratoire de Physiologie et Ecologie MicrobienneUniversite Libre de BruxellesBrusselsBelgium

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