Proteomics of a plant growth-promoting rhizobacterium, Pseudomonas fluorescens MSP-393, subjected to salt shock
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Pseudomonas fluorescens MSP-393, a plant growth-promoting rhizobacterium is an efficient biocontrol agent in rice grown in saline soils of coastal ecosystems. To understand the mechanism of salt tolerance, proteome analysis of the bacterium was carried out employing two-dimensional gel electrophoresis and MALDI-TOF. This technique was used to investigate the regulation of gene product expression of P. fluorescens MSP-393 grown under high osmolarity and used peptide mass fingerprinting and in silico investigation to identify those proteins with altered expression. Among them 15 were assigned to proteins with known functions. Their roles in response to salt stress are discussed.
KeywordsBiocontrol, proteome analysis, Pseudomonas fluorescens, salt stress proteins
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The financial aid from the Department of Biotechnology, Government of India and MALDI-TOF facility from Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India are gratefully acknowledged.
- Anderson M.S., Raetz C.R.H., 1987 Biosynthesis of lipid A precursors in Escherichia coli, a cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine Journal of Biological Chemistry 262:5159–5169Google Scholar
- Buckel S.D., Bell A.W., Mohana Rao J.K., Hermodson M.A. 1986 An analysis of the structure of the product of the rbsA gene of Escherichia coli K12 Journal of Biological Chemistry 261:7659–7662Google Scholar
- Crosa J.H., 1997 Signal transduction and transcriptional and post-transcriptional control of iron-regulated genes in bacteria Microbiology and Molecular Biology Reviews 61:319–336Google Scholar
- Defago G., Berling C.H., Burger U., Haas D., Kahr G., Keel C., Voisard C., Wirthner P., Wurthrich B. 1990 Suppression of black root rot of tobacco and other root diseases by strains of Pseudomonas fluorescens: potential applications and mechanisms In Hornby D., (ed) Biological Control of Soil-Borne Pathogens CAB International Wallingford, UK. pp. 93–108 ISBN 0851986374Google Scholar
- Glaasker E., Heuberger E.H.M.L., Konings W.N., Poolman B., 1998 Mechanism of osmotic activation of the quaternary ammonium compound transporter (Qac T) of L. plantarumJournal of Bacteriology 180:5540–5546Google Scholar
- Kloepper J.W., Schroth M.N., Miller T.D., 1980 Effect of rhizosphere colonization by plant growth promoting rhizobacteria on potato development and yield Phytopathology 70:1078–1082Google Scholar
- Narendra, T. 2005 Unwinding after high salinity stress: development of salinity tolerant plant without affecting yield. ISB News Report, March 2005Google Scholar
- O’Farrell P.H., 1975 High resolution two-dimensional electrophoresis of proteins Journal of Biological Chemistry 250:4007–4021Google Scholar
- Robinson, P.M. 1999 The enzymology of osmoregulation in archaea: glutamine synthetase and β-glutamine production. PhD Thesis Boston College DAI-B 60/06 p. 2677Google Scholar
- Schmalisch M., Langbein I., Stulke J., 2002 The general stress protein Ctc of Bacillus subtilis is a ribosomal protein Journal of Molecular Microbiology and Biotechnology 4:495–501Google Scholar
- Shen S.H., Matsubae M., Takao T., 2002 A proteomic analysis of leaf sheaths from rice Journal of Biochemistry 132:613–620Google Scholar