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Proteomics of a plant growth-promoting rhizobacterium, Pseudomonas fluorescens MSP-393, subjected to salt shock

  • Diby Paul
  • N. Dineshkumar
  • Sudha NairEmail author
Article

Summary

Pseudomonas fluorescens MSP-393, a plant growth-promoting rhizobacterium is an efficient biocontrol agent in rice grown in saline soils of coastal ecosystems. To understand the mechanism of salt tolerance, proteome analysis of the bacterium was carried out employing two-dimensional gel electrophoresis and MALDI-TOF. This technique was used to investigate the regulation of gene product expression of P. fluorescens MSP-393 grown under high osmolarity and used peptide mass fingerprinting and in silico investigation to identify those proteins with altered expression. Among them 15 were assigned to proteins with known functions. Their roles in response to salt stress are discussed.

Keywords

Biocontrol, proteome analysis, Pseudomonas fluorescens, salt stress proteins 

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Notes

Acknowledgements

The financial aid from the Department of Biotechnology, Government of India and MALDI-TOF facility from Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India are gratefully acknowledged.

References

  1. Anderson M.S., Raetz C.R.H., 1987 Biosynthesis of lipid A precursors in Escherichia coli, a cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine Journal of Biological Chemistry 262:5159–5169Google Scholar
  2. Buckel S.D., Bell A.W., Mohana Rao J.K., Hermodson M.A. 1986 An analysis of the structure of the product of the rbsA gene of Escherichia coli K12 Journal of Biological Chemistry 261:7659–7662Google Scholar
  3. Crosa J.H., 1997 Signal transduction and transcriptional and post-transcriptional control of iron-regulated genes in bacteria Microbiology and Molecular Biology Reviews 61:319–336Google Scholar
  4. da Costa M.S., Santos H., Galinski E.A., 1998 An overview of the role and diversity of compatible solutes in Bacteria and Archaea Advances in Biochemical Engineering/Biotechnology 61:117–53CrossRefGoogle Scholar
  5. Defago G., Berling C.H., Burger U., Haas D., Kahr G., Keel C., Voisard C., Wirthner P., Wurthrich B. 1990 Suppression of black root rot of tobacco and other root diseases by strains of Pseudomonas fluorescens: potential applications and mechanisms In Hornby D., (ed) Biological Control of Soil-Borne Pathogens CAB International Wallingford, UK. pp. 93–108 ISBN 0851986374Google Scholar
  6. Fenton W.A., Horwich A.L., 1997 GroEL-mediated protein folding Protein Science 6:743–760CrossRefGoogle Scholar
  7. Glaasker E., Heuberger E.H.M.L., Konings W.N., Poolman B., 1998 Mechanism of osmotic activation of the quaternary ammonium compound transporter (Qac T) of L. plantarumJournal of Bacteriology 180:5540–5546Google Scholar
  8. Hartl F.U., Hlodan R., Langer T., 1994 Molecular chaperones in protein folding: the art of avoiding sticky situations Trends in Biochemical Sciences 19:20–25CrossRefGoogle Scholar
  9. Joanna C.W., Karen A., Homer, Beighton D., 2001 Altered protein expression of Streptococcus oralis cultured at low pH revealed by two-dimensional gel electrophoresis Applied and Environmental Microbiology 67:3396–3405CrossRefGoogle Scholar
  10. Jonathan E.V., Jeffrey K.I., Clarke S., 1998 Mutations in the Escherichia coli surE gene increase isoaspartyl accumulation in a strain lacking the pcm repair methyltransferase but suppress stress-survival phenotypes FEMS Microbiology Letters 167:19–25CrossRefGoogle Scholar
  11. Julianne L., Thomas T., Cavicchioli R., 2000 Low temperature regulated DEAD-box RNA helicase from the antarctic archaeon, Methanococcoides burtonii Journal of Molecular Biology 297:553–567CrossRefGoogle Scholar
  12. Kisseleva L.L., Justesenc J., Alexey D., Wolfson Z.,Yu L. Frolovaa, 1998 Diadenosine oligophosphates (ApnA), a novel class of signalling molecules? FEBS Letters 427:157–163CrossRefGoogle Scholar
  13. Kloepper J.W., Schroth M.N., Miller T.D., 1980 Effect of rhizosphere colonization by plant growth promoting rhizobacteria on potato development and yield Phytopathology 70:1078–1082Google Scholar
  14. Laemmli U.K., 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227:680–685CrossRefGoogle Scholar
  15. Narendra, T. 2005 Unwinding after high salinity stress: development of salinity tolerant plant without affecting yield. ISB News Report, March 2005Google Scholar
  16. O’Farrell P.H., 1975 High resolution two-dimensional electrophoresis of proteins Journal of Biological Chemistry 250:4007–4021Google Scholar
  17. Rangarajan S., Saleena L.M., Vasudevan P., Nair S., 2003 Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions Plant and Soil 251:73–82CrossRefGoogle Scholar
  18. Robinson, P.M. 1999 The enzymology of osmoregulation in archaea: glutamine synthetase and β-glutamine production. PhD Thesis Boston College DAI-B 60/06 p. 2677Google Scholar
  19. Robinson P., Neelon K., Schreier H.J., Roberts M.F., 2001 β-Glutamate as a substrate for glutamine synthetase Applied and Environmental Microbiology 67:4458–4463 CrossRefGoogle Scholar
  20. Schmalisch M., Langbein I., Stulke J., 2002 The general stress protein Ctc of Bacillus subtilis is a ribosomal protein Journal of Molecular Microbiology and Biotechnology 4:495–501Google Scholar
  21. Shen S.H., Matsubae M., Takao T., 2002 A proteomic analysis of leaf sheaths from rice Journal of Biochemistry 132:613–620Google Scholar
  22. Tohru M., Chu R., Yamaguchi S., Macnab R.M., 2000 Role of FliJ in flagellar protein export in Salmonella Journal of Bacteriology 182:4207–4215. CrossRefGoogle Scholar
  23. Volker U., Engelmann S., Maul B., Riethdorf S., Volker A., Schmid R., Mach H., Hecker M., 1994 Analysis of the induction of general stress proteins of Bacillus subtilis Microbiology 140:741–752CrossRefGoogle Scholar
  24. Walt A., Kahn M.L., 2002 The fixA and fixB gene are necessary for anaerobic carnitine reduction in Escherichia coli Journal of Bacteriology 184:4044–4047CrossRefGoogle Scholar
  25. Yale J., Bohnert H.J., 2001 Transcript expression in Saccharomyces cerevisiae at high salinity Journal of Biological Chemistry 276:15996–16007 CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Microbiology GroupM. S. Swaminathan Research FoundationChennaiIndia

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