Proteomics of a plant growth-promoting rhizobacterium, Pseudomonas fluorescens MSP-393, subjected to salt shock

  • Diby Paul
  • N. Dineshkumar
  • Sudha NairEmail author


Pseudomonas fluorescens MSP-393, a plant growth-promoting rhizobacterium is an efficient biocontrol agent in rice grown in saline soils of coastal ecosystems. To understand the mechanism of salt tolerance, proteome analysis of the bacterium was carried out employing two-dimensional gel electrophoresis and MALDI-TOF. This technique was used to investigate the regulation of gene product expression of P. fluorescens MSP-393 grown under high osmolarity and used peptide mass fingerprinting and in silico investigation to identify those proteins with altered expression. Among them 15 were assigned to proteins with known functions. Their roles in response to salt stress are discussed.


Biocontrol, proteome analysis, Pseudomonas fluorescens, salt stress proteins 


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The financial aid from the Department of Biotechnology, Government of India and MALDI-TOF facility from Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India are gratefully acknowledged.


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Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Microbiology GroupM. S. Swaminathan Research FoundationChennaiIndia

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