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Selection and Optimization of Culture Medium for Exopolysaccharide Production by Coriolus (Trametes) Versicolor

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Coriolus versicolor is a medicinal fungus producing exopolysaccharides (EPS). Five well-defined culture media were studied to select the medium that maximizes production of EPS by C. versicolor. Biomass, reducing sugars and EPS concentrations along with the rheological behaviour of the broth were followed during fermentations lasting 9 days. The yeast malt extract medium (YM) was shown to yield the highest production of EPS. Fermentation conditions with YM medium were further investigated to optimize EPS production by C. versicolor. An experimental design to do this was adopted, in which the effects of pH and initial substrate concentration were considered. The effects of initial glucose concentration (5, 15 and 25 g l−1) and pH (4.0, 5.5 and 7.0) were evaluated. The initial glucose concentration was found to be the most important factor in EPS production and also cell growth.

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  1. J-T. Bae J. Sinha J.P. Park C.H. Song J.W. Yun (2000) ArticleTitleOptimization of submerged conditions for exo-biopolymer production by Paecilomyces japonica Journal of Microbiology and Biotechnology 10 482–487 Occurrence Handle1:CAS:528:DC%2BD3cXntFCms7s%3D

  2. A.B. Blakeney P.J. Harris R.J. Henry B.A. Stone (1983) ArticleTitleA simple and rapid preparation of alditol acetates for monosaccharide analysis Carbohydrate Research 113 291–299 Occurrence Handle10.1016/0008-6215(83)88244-5 Occurrence Handle1:CAS:528:DyaL3sXhsl2kurc%3D

  3. G.E.P. Box W.G. Hunter J.S. Hunter (1978) Statistics for Experiments: An Introduction to Design, Data Analysis, and Model Building John Wiley & Sons, Inc New York 306–317

  4. J. Cui Y. Chisti (2003) ArticleTitlePolysccharopeptides of Coriolus versicolor: physiological activity, uses and production Biotechnology Advances 21 109–122 Occurrence Handle10.1016/S0734-9750(03)00002-8 Occurrence Handle1:CAS:528:DC%2BD3sXisV2lu7o%3D

  5. Q.H. Fang J.J. Zhong (2002) ArticleTitleSubmerged fermentation of higher fungus Ganoderma lucidum for production of valuable bioactive metabolites – ganoderic acid and polysaccharide Biochemical Engineering Journal 10 61–65 Occurrence Handle10.1016/S1369-703X(01)00158-9 Occurrence Handle1:CAS:528:DC%2BD38XovFGrtw%3D%3D

  6. P. Khondkar K.E. Aidoo R.F. Tester (2002) ArticleTitleSugar profile of extracellular polysaccharides from different Tremella species International Journal of Food Microbiology 79 121–129 Occurrence Handle10.1016/S0168-1605(02)00186-1 Occurrence Handle1:CAS:528:DC%2BD38XmsVClsbY%3D

  7. T. Kiho J. Hui A. Yamane S. Ukai (1993) ArticleTitlePolysaccharides in fungi. XXXII. Hypoglycemic activity and chemical properties of a polysaccharide from cultural mycelium of Cordyceps sinensis Biological and Pharmaceutical Bulletin 16 1291–1293 Occurrence Handle1:CAS:528:DyaK2cXis1WjsLo%3D

  8. T. Kiho S. Itahashi M. Sakushima T. Matsunaga S. Usui S. Ukai H. Mori H. Sakamoto Y. Ishiguro (1997) ArticleTitlePolysaccharides in fungi. XXXVIII. Anti-diabetic activity and structural feature of a galactomannan elaborated by pestalotiopsis species Biological and Pharmaceutical Bulletin 20 118–121 Occurrence Handle1:CAS:528:DyaK2sXhtlOns70%3D

  9. S.W. Kim H.J. Hwang J.P Park Y.J. Cho C.H. Song J.W. Yun (2002) ArticleTitleMyelial growth and exo-biopolymer production by submerged culture of various edible mushrooms under different media Letters in Applied Microbiology 34 56–61 Occurrence Handle1:CAS:528:DC%2BD38XhsVWmsL0%3D

  10. Y.C. Kuo W.J. Eum M.S. Shiao C.F. Chen C.Y. Lin (1996) ArticleTitleCordyceps sinensis as an immunomodulatory agent American Journal of Chinese Medicine 24 111–125 Occurrence Handle1:STN:280:DyaK2s%2FivVKmtw%3D%3D

  11. J. Lee C. Chung H. Jeong K. Lee (1990) ArticleTitleAnticomplementary and antitumor activities of alkali extract from the mycelia of Lentinus edodes IY 105 Korean Journal of Applied Microbiology and Biotechnology 18 571–577

  12. P.W.A. Mansel (1994) ArticleTitlePolysaccharides in skin care Cosmetics and Toiletries 109 67–72

  13. G.E. Miller (1959) ArticleTitleUse of DNS a reagent for determination of reducings sugars Analytical Chemistry 31 426–428 Occurrence Handle1:CAS:528:DyaG1MXmtFKiuw%3D%3D

  14. T. Miyazaki T. Yadomae M. Sgiura H. Ito K. Fujii S. Naruse M. Kunihisa (1974) ArticleTitleChemical structure of antitumor polysaccharide, coriolan, produced by Coriolus versicolor Chemical and Pharmaceutical Bulletin 22 1739–1742 Occurrence Handle1:CAS:528:DyaE2cXlsFamsbc%3D

  15. T.B. Ng (1998) ArticleTitleA review of research on the protein-bound polysaccharide (polysaccharopeptide, PSP) from the mushroom Coriolus versicolor (Basidiomycetes: Polyporaceae) General Pharmacology 30 1–4 Occurrence Handle1:CAS:528:DyaK2sXntl2htr4%3D

  16. J.P. Park S.W. Kim H.J. Hwang Y.J. Cho Y.S. Lee C.H. Song J.W. Yun (2002) ArticleTitleEffect of agitation intensity on the exo-biopolymer production and mycelial morphology in Cordyceps militaris Letters in Applied Microbiology 34 433–438 Occurrence Handle10.1046/j.1472-765X.2002.01126.x Occurrence Handle1:CAS:528:DC%2BD38XltVyqtrc%3D

  17. B. Roy F. Archibald (1993) ArticleTitleEffects of kraft pulp and lignin on Trametes versicolor carbon metabolism Applied and Environmental Microbiology 59 1855–1863 Occurrence Handle1:CAS:528:DyaK3sXkslCru74%3D

  18. J. Sinha J.T. Bae J.P. Park K.H. Kim C.H. Song J.W. Yun (2001a) ArticleTitleChanges in morphology of Paecilomyces japonica and their effect on broth rheology during production of exo-biopolymers Applied Microbiology and Biotechnology 56 88–92 Occurrence Handle10.1007/s002530100606 Occurrence Handle1:CAS:528:DC%2BD3MXls1Sht74%3D

  19. J. Sinha J.T. Bae J.P. Park C.H. Song J.W. Yun (2001b) ArticleTitleEffect of substrate concentration on broth rheology and fungal morphology during exo-biopolymer production by Paecilomyces japonica in a bioreactor Enzyme and Microbial Technology 29 392–399 Occurrence Handle10.1016/S0141-0229(01)00418-5 Occurrence Handle1:CAS:528:DC%2BD3MXnt1Wgsro%3D

  20. M. Sugiura H. Ohno M. Kunihisa F. Hirata H. Ito (1980) ArticleTitleStudies on antitumor polysaccharides, especially D-II, from mycelium of C. versicolor Japanese Journal of Pharmacology 30 503–513 Occurrence Handle1:CAS:528:DyaL3cXmtlSksb0%3D

  21. A.P.M. Tavares M.A.Z. Coelho J.A.P. Coutinho A.M.R.B. Xavier (2005) ArticleTitleLaccase improvement in submerged cultivation: induced production and kinetic modeling Journal of Chemical Technology and Biotechnology 80 669–676 Occurrence Handle10.1002/jctb.1246 Occurrence Handle1:CAS:528:DC%2BD2MXkvVyruro%3D

  22. M. Tien T.K. Kirk (1988) ArticleTitleLignin peroxidase of Phanerochaete chrysosporium Methods in Enzymology 161 238–249 Occurrence Handle1:CAS:528:DyaL1MXhs1yqtbo%3D Occurrence Handle10.1016/0076-6879(88)61025-1

  23. L. Vuyst ParticleDe (2000) ArticleTitleTechnology aspects related to the application of functional starter cultures Food Technology and Biotechnology 38 105–112

  24. M.J. Waites N.L. Morgan J.S. Rockey A.G. Hi Gton (2001) Industrial Microbiology, An Introduction Blackwell Publishing Oxford 68

  25. R.L. Whistler J.N. BeMiller (1997) Carbohydrate Chemistry for Food Scientists Eagan Press St. Paul 96

  26. B.K. Yang J.Y. Ha S.C. Jcong Y.J. Jcon K.S. Ra S. Das J.W. Yun C.H. Song (2002) ArticleTitleHypolipidemic effect of an exo-biopolymer produced from submerged mycelial cultures of Auricularia polytricha in rats Biotechnology Letters 24 1319–1325 Occurrence Handle1:CAS:528:DC%2BD38Xmt12rurg%3D

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Correspondence to A. M. R. B. Xavier.

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Tavares, A.P.A., Agapito, M.S.M., Coelho, M.A.M. et al. Selection and Optimization of Culture Medium for Exopolysaccharide Production by Coriolus (Trametes) Versicolor . World J Microbiol Biotechnol 21, 1499–1507 (2005).

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  • Coriolus versicolor
  • exopolysaccharide production
  • experimental design
  • optimization
  • submerged culture