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Selection and Optimization of Culture Medium for Exopolysaccharide Production by Coriolus (Trametes) Versicolor

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Summary

Coriolus versicolor is a medicinal fungus producing exopolysaccharides (EPS). Five well-defined culture media were studied to select the medium that maximizes production of EPS by C. versicolor. Biomass, reducing sugars and EPS concentrations along with the rheological behaviour of the broth were followed during fermentations lasting 9 days. The yeast malt extract medium (YM) was shown to yield the highest production of EPS. Fermentation conditions with YM medium were further investigated to optimize EPS production by C. versicolor. An experimental design to do this was adopted, in which the effects of pH and initial substrate concentration were considered. The effects of initial glucose concentration (5, 15 and 25 g l−1) and pH (4.0, 5.5 and 7.0) were evaluated. The initial glucose concentration was found to be the most important factor in EPS production and also cell growth.

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Correspondence to A. M. R. B. Xavier.

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Tavares, A.P.A., Agapito, M.S.M., Coelho, M.A.M. et al. Selection and Optimization of Culture Medium for Exopolysaccharide Production by Coriolus (Trametes) Versicolor . World J Microbiol Biotechnol 21, 1499–1507 (2005). https://doi.org/10.1007/s11274-005-7370-7

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Keywords

  • Coriolus versicolor
  • exopolysaccharide production
  • experimental design
  • optimization
  • submerged culture