Growth and Starvation of a Strain of Klebsiella pneumoniae Isolated from a Brazilian Oil Formation
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Summary
The changes that occur in cell size and shape during experimental starvation and resuscitation of bacteria may find practical application in the biotechnological processes used in microbial enhanced oil-recovery (MEOR), in in situ bioremediation and in the creation of biobarriers. In the work described here, the aim was to observe certain aspects of the response of a strain of Klebsiella pneumoniae, isolated from oil producer wells belonging to PETROBRAS (National Oil Company of Brazil), to starvation and growth under various culture conditions. Cells of K. pneumoniae were taken from an exponentially-growing culture, washed and suspended in phosphate-buffered saline solution, without nutrients, where they remained for 91 days. Aliquots were withdrawn periodically, to perform viable and total cell counts and measure the optical density and cell dimensions. The starved cells were resuspended in sodium citrate medium, where they recuperated and exhibited a typical growth curve. The results indicate that this strain is a viable option for future trials on the transport and growth of microorganisms inside porous media, aimed at possible applications in MEOR.
Keywords
cell size image analysis Klebsiella pneumoniae MEOR starvationPreview
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References
- Amy, P.S., Morita, R.Y. 1983Starvation-survival patterns of sixteen freshly isolated open-ocean bacteriaApplied and Environmental Microbiology4511091115Google Scholar
- Amy, P.S., Pauling, C., Morita, R. 1983Recovery from nutrient starvation by a marine Vibrio spApplied and Environmental Microbiology4516851690Google Scholar
- Bogosian, G., Morris, P.J.L., O’Neil, J.P. 1998A mixed culture recovery method indicates that enteric bacteria do not enter the viable but nonculturable stateApplied and Environmental Microbiology6417361742Google Scholar
- Bolter, M., Möller, R., Dzomla, W. 1993Determination of bacterial biovolume with epifluorescence microscopy: comparison of size distributions from image analysis and size classificationsMicron243140Google Scholar
- Borzani, W. 1993Objective criteria to confirm the existence of the exponential growth phase in a batch microbial processRevista de Microbiologia24278280Google Scholar
- Bryers, J.D., Sanin, S. 1994Resuscitation of starved ultramicrobacteria to improve in situ bioremediationAnnals of the New York Academy of Sciences7456176Google Scholar
- Caccavo, F.,Jr, Ramsing, N.B., Costerton, J.W. 1996Morphological and metabolic responses to starvation by the dissimilatory metal-reducing bacterium Shewanella alga BrYApplied and Environmental Microbiology6246784682Google Scholar
- Collins, C.H., Lyne, P.M., Grange, J.M. 1995Collin’s and Lyne’s Microbiological MethodsButterworth – HeinemannOxfordISBN 0–7506-0653-3Google Scholar
- Cusack, F.M., Singh, S., Mccarthy, C., Grieco, J., Rocco, M., Nguyen, D., Lappin-Scott, H., Costerton, J.W. 1992Enhanced oil recovery – three dimensional sandpack simulation of ultramicrobacteria resuscitation in reservoir formationJournal of General Microbiology138647655Google Scholar
- Joux, F., Lebaron, P., Troussellier, M. 1997Succession of cellular states in a Salmonella typhimurium population during starvation in artificial seawater microcosmsFEMS Microbiology Ecology226576CrossRefGoogle Scholar
- Kepner, R.L., Pratt, J.R. 1994Use of fluorochromes in direct enumeration of total bacteria in environmental samples: past and presentMicrobiological Reviews58603615Google Scholar
- Kurath, G., Morita, R.Y. 1983Starvation-survival physiological studies of a marine Pseudomonas spApplied and Environmental Microbiology4512061211Google Scholar
- Lappin-Scott, H., Costerton, J.W. 1990Starvation and penetration of bacteria in soils and rocksExperientia46807812CrossRefGoogle Scholar
- Lappin-Scott, H., Cusack, F., Macleod, A., Costerton, J.W. 1988Starvation and nutrient resuscitation of Klebsiella isolated from oil well watersJournal of Applied Bacteriology64541549Google Scholar
- Macleod, F.A., Lappin-Scott, H.M., Costerton, J.W. 1988Plugging of a model rock system by using starved bacteriaApplied and Environmental Microbiology5413651372Google Scholar
- Mason, C.A., Hamer, G. 1987Cryptic growth in Klebsiella pneumoniae Applied Microbiology and Biotechnology25577584Google Scholar
- Massana, R., Gasol, J.M., Bjørsen, P.K., Black-Burn, N., Hagström, A., Hietanen, S., Hygum, B.H., Kuparinen, J., Pedrós-Alió, C. 1997Measurement of bacterial size via image analysis of epifluorescence preparations: description of an inexpensive system and solutions to some of the most common problemsScientia Marina61397407Google Scholar
- Media Cybernetics. 1997 Image-Pro Plus ver. 3.0. Silver Spring, USA: Media Cybernetics. 1 CD-ROMGoogle Scholar
- Morita, R.Y. 1988Bioavailability of energy and its relationship to growth and starvation survival in natureCanadian Journal of Microbiology34436441CrossRefGoogle Scholar
- Morita, R.Y. 1990The starvation-survival state of microorganisms in nature and its relationship to the bioavailable energyExperientia46813817CrossRefGoogle Scholar
- Morita, R.Y. 1993Bioavailability of energy and the starvation stateKjelleberg, S. eds. Starvation in BacteriaPlenum PressNew York123ISBN 0-306-44430-5Google Scholar
- Moyer, C.L., Morita, R.Y. 1989Effect of growth rate and starvation-survival on cellular DNA, RNA, and protein of a psychrophilic marine bacteriumApplied and Environmental Microbiology5527102716Google Scholar
- Novitsky, J.A., Morita, R.Y. 1976Morphological characterization of small cells resulting from nutrient starvation of a psychrophilic marine Vibrio Applied and Environmental Microbiology32617622Google Scholar
- Shaw, J.C., Bramhill, B., Wardlaw, N.C., Costerton, J.W. 1985Bacterial fouling in a model core systemApplied and Environmental Microbiology49693701Google Scholar
- Tabor, P.S., Ohwada, K., Colwell, R.R. 1981Filterable marine bacteria found in the deep sea: distribution, taxonomy, and response to starvationMicrobial Ecology76783Google Scholar
- Torrella, F., Morita, R.Y. 1981Microcultural study of bacterial size changes and microcolony and ultramicrocolony formation by heterotrophic bacteria in seawaterApplied and Environmental Microbiology41518527Google Scholar
- Zar, J.H. 1984Biostatistical Analysis2Prentice HallNew JerseyISBN 0-13077925-3Google Scholar
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