World Journal of Microbiology and Biotechnology

, Volume 21, Issue 8–9, pp 1409–1414 | Cite as

Identification and Kinetic Characteristics of an Indigenous Diesel-degrading Gordonia alkanivorans Strain

  • Chiu-Chung Young
  • Ta-Chen Lin
  • Mao-Song Yeh
  • Fo-Ting Shen
  • Jo-Shu ChangEmail author


An indigenous strain Gordonia alkanivorans CC-JG39 was isolated from oil-contaminated sludge of a local gas station located in central Taiwan. The bacterial isolate was able to grow on diesel-containing Bushnell–Haas medium and also tolerate various chemical additives frequently used in petroleum products (e.g. BETX, methyl-tert-butyl ether, and naphthalene). Kinetics of diesel-limited cell growth and biodegradation of diesel followed a Monod-type model. The kinetic constants for cell growth (μmax and KS,G) were 0.158 h−1 and 3196 mg/l, respectively, while those for biodegradation of diesel (vmax, diesel and KS,D) were 3.59 mg/h/mg cell and 2874 mg/l, respectively. G. alkanivorans CC-JG39 produced extracellular surface-active material, leading to a low surface tension of nearly 33 mN/m. The CC-JG39 strain also possessed the ability to float towards the oil/water interface. These features might play some roles in enhancing the mass transfer efficiency between oil substrate and the bacterial cells. Therefore, G. alkanivorans CC-JG39 may have potential applications in bioremediation of oil pollution sites.


Biodegradation biosurfactant diesel oil floating activity Gordonia alkanivorans 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abalos, A., Pinazo, A., Infante, M.R., Casals, M., García, F., Manresa, A. 2001Physicochemical and antimicrobial properties of new rhamnolipids produced by Pseudomonas aeruginosa AT10 from soybean oil refinery wastesLangmuir1713671371CrossRefGoogle Scholar
  2. Banat, I.M. 1995Biosurfactants production and possible uses in microbial enhanced oil recovery and oil pollution remediation: a reviewBioresource Technology51112CrossRefGoogle Scholar
  3. Belleman, P., Bereswill, S., Berger Geider, S. K. 1994Visualization of capsule formation by Erwinia amylovora and assay to determine amylovoran synthesisInternational Journal of Biological Macromolecules16290296Google Scholar
  4. Bosch, M.O., Robert, M., Mercadé, M.E., Espuny, M.J., Parra, J.L., Guinea, J. 1988Surface active compounds on microbial culturesTenside Surfactants Detergents25208211Google Scholar
  5. Brosius, J., Palmer, M.L., Kennedy, P.J., Noller, H.R. 1978Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coliProceedings of the National Academy of Sciences of the United States of America7548014805Google Scholar
  6. Bushnell, L.D., Haas, H.F. 1941The utilization of certain hydrocarbons by microorganismsJournal of Bacteriology41653673Google Scholar
  7. Chaillan, F., Le Fleche, A., Bury, E., Phantavong, Y.H., Grimont, P., Saliot, A., Oudot, J. 2004Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganismsResearch in Microbiology155587595CrossRefGoogle Scholar
  8. Edwards, U., Rogall, T., Blöcker, H., Emde, M., Böttger, E.C. 1989Isolation and direct complete nucleotide determination of entire genes Characterization of a gene coding for 16S ribosomal RNANucleic Acids Research1778437853Google Scholar
  9. Grangemard, I., Wallach, J., Maget-dana, R., Peypoux, F. 2001A more efficient cation chelator than surfactinApplied Biochemistry and Biotechnology90199210CrossRefGoogle Scholar
  10. Iwahori, K., Tokutomi, T., Miyata, N., Fujita, M. 2001Formation of stable foam by the cells and culture supernatant of Gordonia (Nocardia) amaraeJournal of Bioscience and Bioengineering927779Google Scholar
  11. Kim, D., Kim, Y.S., Kim, S.K., Kim, S.W., Zylstra, G.J., Kim, Y.M., Kim, E. 2002Monocyclic aromatic hydrocarbon degradation by Rhodococcus sp. strain DK17Applied and Environmental Microbiology6832703278Google Scholar
  12. Lin, T.C., Young, C.C., Ho, M.J., Yeh, M.S., Chou, C.L., Wei, Y.H. & Chang, J.S. 2005 Characterization of floating activity of indigenous diesel-assimilating bacterial isolates. Journal of Bioscience and Bioengineering, in press.Google Scholar
  13. Pagilla, K.R., Sood, A., Kim, H. 2002Gordonia (nocardia) amarae foaming due to biosurfactant productionWater Science and Technology46519524Google Scholar
  14. Ron, E.Z., Rosenberg, E. 2002Biosurfactant and oil bioremediationCurrent Opinion in Biotechnology13249252CrossRefGoogle Scholar
  15. Taylor, B.D. 1988Environmental regulations place new responsibilities on property ownersCalabrese, E.J.Kostecki, P.T. eds. Soils Contaminated by Petroleum: Environmental and Public Health EffectsWileyNew York, Chichester37ISBN 047185106XGoogle Scholar
  16. Thomassin-Lacroix, E.J.M., Eriksson, M., Reimer, K.J., Mohn, W.W. 2002Biostimulation and bioaugmentation for on-site treatment of weathered diesel fuel in Arctic soilApplied Microbiology and Biotechnology59551556Google Scholar
  17. Villareal, T.A., Carpenter, E.J. 2003Buoyancy regulation and the potential for vertical migration in the oceanic cyanobacterium trichodesmiumMicrobial Ecology45110CrossRefGoogle Scholar
  18. Vinas, M., Grifoll, M., Sabate, J., Solanas, A.M. 2002Biodegradation of a crude oil by three microbial consortia of different origins and metabolic capabilitiesJournal of Industrial Microbiology and Biotechnology28252260Google Scholar
  19. Watts, D., MacBeath, J.R. 2001Automated fluorescent DNA sequencing on the ABI PRISM 310 Genetic AnalyzerMethods in Molecular Biology16715317Google Scholar
  20. Wolfaardt, G.M., Lawrence, J.R., Headley, J.V., Robarts, R.D. 1994Microbial exopolymers provide a mechanism for bioaccumulation of contaminantsMicrobial Ecology27279291CrossRefGoogle Scholar
  21. Xue, Y.F., Sun, X.S., Zhou, P. J., Liu, R.L., Liang Ma, F.L.Y.H. 2003Gordonia paraffinivorans sp nov., a hydrocarbon-degrading actinomycete isolated from an oil-producing wellInternational Journal of Systematic and Evolutionary Microbiology5316431646CrossRefGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Chiu-Chung Young
    • 1
  • Ta-Chen Lin
    • 2
  • Mao-Song Yeh
    • 3
  • Fo-Ting Shen
    • 1
  • Jo-Shu Chang
    • 3
    Email author
  1. 1.Department of Soil and Environmental SciencesNational Chung Hsing UniversityTaichungTaiwan
  2. 2.Department of Environmental EngineeringNational Chung Hsing UniversityTaichungTaiwan
  3. 3.Department of Chemical EngineeringNational Cheng Kung UniversityTainanTaiwan

Personalised recommendations