A novel aqueous two-phase system containing hydrophobically modified ethylene oxide (HM-EO) and sodium dodecyl sulphate (SDS) was developed to enhance the selectivity of protein partitioning in two phases. Phase diagrams of HM-EO/H2O and HM-EO/SDS/H2O were measured, and the mechanism of interaction between HM-EO polymer and the anionic surfactant sodium dodecyl sulphate (SDS) was also discussed. It was found that the improvement of selectivity of protein partitioning was related to the increase of electrostatic potential difference between the two phases because of the charged network formed by mixed micelles of HM-EO and SDS in the bottom phase. With bovine serum albumin (BSA) and lysozyme as model proteins, some factors, such as pH, SDS concentration, conductivity and temperature of the system, were investigated for the influences of protein partition in HM-EO/SDS/H2O systems. The results showed that the addition of SDS not only changed the phase behaviour, but also played an important role in protein partitioning.
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E. Alami M. Almgren W. Brown (1996a) ArticleTitleInteraction of hydrophobically end-capped poly(ethylene oxide) with nonionic surfactants in aqueous solution: fluorescence and light-scattering studies Macromolecules 29 5026–5035 Occurrence Handle1:CAS:528:DyaK28XjsVaisbk%3D
E. Alami M. Almgren W. Brown (1996b) ArticleTitleAggregation of hydrophobically end-capped poly(ethylene oxide) in aqueous solutions: fluorescence and Light-Scattering and light-scattering studies Macromolecules 29 2229–2243 Occurrence Handle1:CAS:528:DyaK28XhtFeku7Y%3D
P.-A. Albertsson (1986) Partitioning of Cell Particles and Macromolecules EditionNumber3 Wiley New York (USA)
F. Guillemet L. Piculell (1995) ArticleTitleInteractions in aqueous mixtures of hydrophobically modified polyelectrolyte and oppositely charged surfactant: mixed micelle formation and associative phase separation The Journal of Physical Chemistry 99 9201–9209 Occurrence Handle10.1021/j100022a038 Occurrence Handle1:CAS:528:DyaK2MXlslyrtbw%3D
U. Gunduz K. Korkmaz (2000) ArticleTitleBovine serum albumin partitioning in an aqueous two-phase system: effect of pH and sodium chloride concentration Journal of Chromatography B 743 255–258 Occurrence Handle1:CAS:528:DC%2BD3cXktlKmtro%3D
H.-O. Johansson J. Persson F. Tjerneld (1999) ArticleTitleThermoseparating water/polymer system: a novel one-polymer aqueous two-phase system for protein purification Biotechnology and Bioengineering 66 247–257 Occurrence Handle10.1002/(SICI)1097-0290(1999)66:4<247::AID-BIT6>3.0.CO;2-5 Occurrence Handle1:CAS:528:DyaK1MXotFalsr8%3D
M. Jonsson H.-O. Johansson (2003) ArticleTitleProtein partitioning in thermoseparating systems of a charged hydrophobically modified ethylene oxide polymer Journal of Chromatography A 983 133–134 Occurrence Handle10.1016/S0021-9673(02)01695-3 Occurrence Handle1:CAS:528:DC%2BD38XpsFejtrc%3D
M. Karlberg K. Thuresson L. Piculell B. Lindman (2004) ArticleTitleMixed solutions of hydrophobically modified graft and block copolymers Colloids and Surfaces A: Physicochemical and Engineering Aspects 236 159–164 Occurrence Handle10.1016/j.colsurfa.2004.01.035 Occurrence Handle1:CAS:528:DC%2BD2cXjt1WgtLw%3D
J.-W. Li (1994) The Principles and Methods of Biochemical Experiments Beijing University Press BeiJing (P.R. China)
L. Piculell F. Guillemet K. Thuresson V. Shubin O. Ericsson (1996) ArticleTitleBinding of surfactants to hydrophobically modified polymers Advances in Colloid and Interface Science 63 1–21 Occurrence Handle10.1016/0001-8686(95)00272-3 Occurrence Handle1:CAS:528:DyaK28Xktl2qtQ%3D%3D
V. Seredyuk M. Nyden K. Holmberg A.V. Peresypkin F.M. Menger (2001) ArticleTitleMicellization and adsorption properties of novel zwitterionic surfactants Langmuir 17 5160–5165 Occurrence Handle10.1021/la010182q Occurrence Handle1:CAS:528:DC%2BD3MXltlSqt74%3D
F. Tanaka T. Koga (2000) ArticleTitleIntramolecular and intermolecular association in thermoreversible gelation of hydrophobically modified associating polymers Computational and Theoretical Polymer Science 10 259–267 Occurrence Handle1:CAS:528:DC%2BD3cXjtFWjsLY%3D
K. Thuresson L. Karlson B. Lindman (2002) ArticleTitleClouding of a cationic hydrophobically associating comb polymer Colloids and Surfaces A: Physicochemical and Engineering Aspects 201 9–15 Occurrence Handle10.1016/S0927-7757(00)00775-5 Occurrence Handle1:CAS:528:DC%2BD38XhsVagsb8%3D
C. Tribet (1998) ArticleTitleHydrophobically driven attachments of synthetic polymer onto surfaces of biological interest: Lipid bilyers and globular porteins Biochimie 80 461–473 Occurrence Handle1:CAS:528:DyaK1cXmt1Sntbc%3D
B. Xu A. Yekta M.A. Winnik (1997) ArticleTitleViscoelastic properties in water of comb associative polymers based on poly(ethylene oxide) Langmuir 13 6903–6911 Occurrence Handle1:CAS:528:DyaK1cXjtlGq
G.-X. Zhao (1991) Physical Chemistry of Surfactants EditionNumber2 Beijing University Press. Beijing (P.R. -China)
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Deng, G., Yao, S. & Lin, D. Partitioning of Proteins using a Hydrophobically Modified Ethylene Oxide/SDS Aqueous Two-phase System. World J Microbiol Biotechnol 21, 1209–1214 (2005). https://doi.org/10.1007/s11274-005-1442-6
- Aqueous two-phase system
- hydrophobically modified ethylene oxide
- mixed micellar aggregates
- phase diagram
- protein partitioning