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Phosphatidylinositol-Specific Phospholipase C Production from Bacillus thuringiensis Serovar. kurstaki using Potato-Based Media

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Summary

Few studies on screening of the enzyme phosphatidylinositol-specific phospholipase C (PI-PLC) have been reported. Here we present a simple potato-based medium to elaborate large quantities of extracellular PI-PLC from Bacillus thuringiensis serovar. kurstaki. The enzyme activity (1.75 U/ml) was found to be maximum in the potato-extract medium supplemented with sucrose and mineral salts with a specific activity of 3.88 U/mg protein.

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Correspondence to T. Palvannan.

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Palvannan, T., Boopathy, R. Phosphatidylinositol-Specific Phospholipase C Production from Bacillus thuringiensis Serovar. kurstaki using Potato-Based Media. World J Microbiol Biotechnol 21, 1153–1155 (2005). https://doi.org/10.1007/s11274-005-0299-z

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Keywords

  • Acetylcholinesterases
  • Bacillus thuringiensis
  • phosphatidylinositol-specific phospholipases C
  • screening
  • triacyl-glycerol