Few studies on screening of the enzyme phosphatidylinositol-specific phospholipase C (PI-PLC) have been reported. Here we present a simple potato-based medium to elaborate large quantities of extracellular PI-PLC from Bacillus thuringiensis serovar. kurstaki. The enzyme activity (1.75 U/ml) was found to be maximum in the potato-extract medium supplemented with sucrose and mineral salts with a specific activity of 3.88 U/mg protein.
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M.J. Berridge R.F. Irvine (1984) ArticleTitleInositol trisphosphate, a novel second messenger in cellular signal transduction Nature 312 315–321 Occurrence Handle10.1038/312315a0 Occurrence Handle1:CAS:528:DyaL2MXhvFKktw%3D%3D
C. Bordier (1981) ArticleTitlePhase separation of integral membrane proteins I Triton X-114 solution Journal of Biological Chemistry 256 1604–1607 Occurrence Handle1:CAS:528:DyaL3MXhsFaqurg%3D
K. Clausen (2001) ArticleTitleEnzymatic oil-degumming by a novel microbial phospholipase European Journal of Lipid Science and Technology 103 333–340 Occurrence Handle10.1002/1438-9312(200106)103:6<333::AID-EJLT333>3.3.CO;2-6 Occurrence Handle1:CAS:528:DC%2BD3MXkslWnsLw%3D
G.L. Ellman D.K. Courtney V. Anders R.M. Fetherstone (1961) ArticleTitleA new and rapid colorimetric determination of acetylcholinesterase activity Biochemical Pharmacology 7 88–95 Occurrence Handle10.1016/0006-2952(61)90145-9 Occurrence Handle1:CAS:528:DyaF3MXht1Gns7o%3D
O. Kirk T.V. Borchert C.C. Fuglsang (2002) ArticleTitleIndustrial enzyme applications Current Opinion in Biotechnology 13 345–351 Occurrence Handle10.1016/S0958-1669(02)00328-2 Occurrence Handle1:CAS:528:DC%2BD38XmsFOnsLs%3D
Y. Ohno (2002) ArticleTitleDeep-frying oil properties of diacylglycerol-rich cooking oil Journal of Oleo Science 51 275–279 Occurrence Handle1:CAS:528:DC%2BD38Xitl2rsLw%3D
S. Poopathi K. Anup kumar L. Kabilan V. Sekar (2002) ArticleTitleDevelopment of low-cost media for the culture of mosquito larvicides, Bacillus sphaericus and Bacillus thuringiensis serovar. israelensis World Journal of Microbiology and Biotechnology 18 209–216 Occurrence Handle10.1023/A:1014937311839
S.G. Rhee Y.S. Bae (1997) ArticleTitleRegulation of phosphoinositide specific phospholipase C isozymes Journal of Biological Chemistry 272 15045–15048 Occurrence Handle1:CAS:528:DyaK2sXjvFOns70%3D
M.G. Soni H. Kimura G.A. Burdock (2001) ArticleTitleChronic study of diacylglycerol oils in rat Food Chemistry and Toxicology 39 317–329 Occurrence Handle1:CAS:528:DC%2BD3MXisF2hurY%3D
J.G. Songer (1997) ArticleTitleBacterial phospholipases and their role in virulence Trends in Microbiology 5 156–161 Occurrence Handle10.1016/S0966-842X(97)01005-6 Occurrence Handle1:STN:280:ByiB1crpvVY%3D
R. Taguchi K. Suzuki T. Nakabayashi H. Ikezawa (1984) ArticleTitleAcetylcholinesterase release from mammalian erythrocytes by phosphatidylinositol-specific phospholipase C of Bacillus thuringiensis and characterization of the released enzyme Journal of Biochemistry 96 437–446 Occurrence Handle1:CAS:528:DyaL2cXlt1Gls74%3D
K. Yamamoto H. Asakawa K. Tokunaga H. Watanabe N. Matsuo I. Tokimitsu N. Yagi (2000) ArticleTitleLong-term ingestion of dietary diacylglycerol lowers serum triacylglycerol in type II diabetic patients with hypertriglyceridemia Journal of Nutrition 131 3204–3207
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Palvannan, T., Boopathy, R. Phosphatidylinositol-Specific Phospholipase C Production from Bacillus thuringiensis Serovar. kurstaki using Potato-Based Media. World J Microbiol Biotechnol 21, 1153–1155 (2005). https://doi.org/10.1007/s11274-005-0299-z
- Bacillus thuringiensis
- phosphatidylinositol-specific phospholipases C