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World Journal of Microbiology and Biotechnology

, Volume 21, Issue 6–7, pp 1119–1122 | Cite as

Lactase Production from Lactobacillus acidophilus

  • S. K. Akolkar
  • A. Sajgure
  • S. S. LeleEmail author
Article

Summary

A lactobacillus strain isolated from fermented Ragi (Eleusine coracana Gaertn.) was characterized as Lactobacillus acidophilus. The isolate was found to be homofermentative, slime-forming and a lactase (β-galactosidase) producer. Production, recovery, characterization and performance of lactase were studied at laboratory scale from 100 ml to 5 l under stationary and stirred conditions. 1.5% lactose was found to be the best carbon source for lactase production. The lactose content could be reduced to 0.75% by supplementing with 1% ragi, thus making the media economically more attractive. A 6.5-fold increase (5400 U ml−1) was achieved on scale-up. Performance of the lactase obtained from this strain was found to be slightly better than the commercial lactase produced by Kluyveromyces lactis.

Keywords

Eleusine coracana lactase Lactobacillus acidophilus scale-up yield optimization 

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Food and Fermentation Technology DepartmentUniversity Institute of Chemical TechnologyMatunga, MumbaiIndia

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