Isolation and characterization of actinomycetes antagonistic to pathogenic Vibrio spp. from nearshore marine sediments
A total of 94 actinomycete strains were isolated from the marine sediments of a shrimp farm, 87.2% belonged to the genus Streptomyces, others were Micromonospora spp. Fifty-one percent of the actinomycete strains showed activity against the pathogenic Vibrio spp. strains. Thirty-eight percent of marine Streptomyces strains produced siderophores on chrome azurol S (CAS) agar plates. Seven strains of Streptomyces were found to produce siderophores and to inhibit the growth of Vibrio spp. in vitro. Two of them belonged to the Cinerogriseus group, the most frequently isolated group of Streptomyces. The results showed that streptomycetes could be a promising source for biocontrol agents in aquaculture.
KeywordsMarine actinomycetes probiotics siderophores Vibrio
Barcina, I., Iriberri, J., Egea, L. 1987Enumeration, isolation and some physiological properties of actinomycetes from sea water and sedimentSystematic and Applied Microbiology108591Google Scholar Bhashkar, N., Setty, T.M.R., Mondal, S., Joseph, M.A., Raju, C.V., Raghunath, B.S., Anantha, C.S. 1998Prevalence of bacteria of public health significance in the cultured shrimp (Penaeus monodon)Food Microbiology15511519CrossRefGoogle Scholar Clark, V.L., Bavoil, P.M. 1994Methods in Enzymology 235(A)Academic PressLondon315372Google Scholar Eleonor, A.T., Leobert, D., Pena, 2001Antibiotic resistance of bacteria from shrimp pondsAquaculture195193204CrossRefGoogle Scholar Emmert, A.B.E., Handelsman, J. 1999Biocontrol of plant disease: a (Gram-) positive perspectiveFEMS Microbiology Letters17119CrossRefPubMedGoogle Scholar Goarant, C., Merien, F., Berthe, F., Mermoud, I., Perolat, P. 1999Arbitrarily primed PCR to type Vibrio spp. pathogenic for ShrimpApplied and Environmental Microbiology6511451151PubMedGoogle Scholar Goodfellow, M., Cross, T. 1984ClassificationGoodfellow, M.Mordarski, M.Williams, S.T. eds. The Biology of the ActinomycetesAcademic PressLondon7164Google Scholar Gram, L., . Melchiorsen, J., Spanggaard, B., Huber, I., Nielsen, T.F. 1999Inhibition of Vibrio anguillarum by Pseudomonas fluorescens AH2, a possible probiotic treatment of fishApplied and Environmental Microbiology65969973PubMedGoogle Scholar Guerinot, M.L. 1994Microbial iron transportAnnual Review of Microbiology48743772CrossRefPubMedGoogle Scholar Jensen, P.R., Dwight, R., Fenical, W. 1991Distribution of actinomycetes in near-shore tropical marine sedimentsApplied and Environmental Microbiology2911021108Google Scholar Skjermo, J., Vadstein, O. 1999Techniques for microbial control in the intensive rearing of marine larvaeAquaculture177333343CrossRefGoogle Scholar Sung, H.H., Hsu, S.F., Chen, C.K., Ting, Y.Y., Chao, W.L. 2001Relationships between disease outbreak in cultured tiger shrimp (Penaeus monodon) and the composition of Vibrio communities in pond water and shrimp hepatopancreas during cultivationAquaculture192101110CrossRefGoogle Scholar Takizawa, M., Colwell, R.R., Hell, R.T. 1993Isolation and diversity of actinomycetes in the Chesapeake bayApplied and Environmental Microbiology599971002Google Scholar Urakawa, H., Kita-Tsukamoto, K., Ohwada, K. 1999Microbial diversity in marine sediments from Sagami Bay and Tokyo Bay, Japan, as determined by 16S rRNA gene analysisMicrobiology14533053315PubMedGoogle Scholar Verschuere, L., Rombaut, G., Sorgeloos, P., Verstraete, W. 2000Probiotic bacteria as biological control agents in aquacultureMicrobiology and Molecular Biology Reviews64655671CrossRefPubMedGoogle Scholar Zheng, Z.H., Zeng, W., Huang, Y.J., Yang, Z.Y., Li, J., Cai, H.R., Su, W.J. 2000Detection of antitumor and antimicrobial activities in marine organism associated actinomycetes isolated from the Taiwan Straint, ChinaFEMS Microbiology Letters1888791CrossRefPubMedGoogle Scholar