Summary
Studies conducted for the multiplication of Bacillus thuringiensis (Bt) using barley Hordeum vulgare as the carbon source led to the development of a protocol for the cost-effective, mass production of Bt. The production employs the simple shake flask method and can be easily adopted with a production potential of 1.5 kg Bt per day approximately at an overall production cost of Rs. 360 kg-1 (8 US dollars). The protocol is suitable for promoting localized production of Bt at the village/district level. The product when tested as 0.1% (w/v) spray against the castor semilooper, Achoea janata proved highly effective, causing immediate feeding cessation of the larvae followed by 85% and 100% mortality by 48 and 72 h after treatment, respectively.
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Vimala Devi, P.S., Ravinder, T. & Jaidev, C. Barley-based medium for the cost-effective production of Bacillus thuringiensis. World J Microbiol Biotechnol 21, 173–178 (2005). https://doi.org/10.1007/s11274-004-2217-1
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DOI: https://doi.org/10.1007/s11274-004-2217-1