Optimization of carotenoid production by Rhodotorula glutinis using statistical experimental design


DOI: 10.1007/s11274-004-1891-3

Cite this article as:
Park, P.K., Cho, D.H., Kim, E.Y. et al. World J Microbiol Biotechnol (2005) 21: 429. doi:10.1007/s11274-004-1891-3


A two-step optimization strategy of statistical experimental design was employed to enhance carotenoid production from sugar cane molasses (SCM) in the yeast Rhodotorula glutinis. In the first step, a fractional factorial design was used to evaluate the impact of five fermentation factors (pH and concentrations of SCM, urea, KH2PO4, and NaCl). The results revealed that three factors (concentrations of SCM, urea, and KH2PO4) had a significant influence on biomass and carotenoid production. A face-centered central composite design was then used in the second step to optimize the three significant factors to further enhance the biomass yield and carotenoid production. Through this two-step optimization strategy, the carotenoid concentration could be increased from an average of 1.39 mg/l to an average of 3.46 mg/l, representing a 2.5-fold carotenoid production enhancement.


Carotenoids factorial design medium optimization Rhodotorula glutinis 

Copyright information

© Springer 2005

Authors and Affiliations

  • P. K. Park
    • 1
  • D. H. Cho
    • 1
  • E. Y. Kim
    • 1
  • K. H. Chu
    • 2
  1. 1.Department of Chemical EngineeringThe University of SeoulSeoulKorea
  2. 2.Department of Chemical and Process EngineeringUniversity of CanterburyChristchurchNew Zealand

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